Evidence map›Paper›PMID 41249819›Full record

ArticleNature structural & molecular biology2025

ATG2A-mediated DAG transfer recruits DGAT2 for lipid droplet growth.

Helin Elhan, Alicia Damm, Justin L Korfhage, Daniel Álvarez, Mehdi Zouiouich, Francesca Giordano, Stefano Vanni, Thomas J Melia, Abdou Rachid Thiam

Abstract read
In one paragraph

Article in Nature structural & molecular biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Helin ElhanLaboratoire de Physique de l'École Normale Supérieure, Université PSL, CNRS, Sorbonne Université, Université de Paris, Paris, France.
Alicia DammLaboratoire de Physique de l'École Normale Supérieure, Université PSL, CNRS, Sorbonne Université, Université de Paris, Paris, France.
Justin L KorfhageDepartment of Cell Biology, Yale University School of Medicine, New Haven, CT, USA.
Daniel ÁlvarezDepartment of Biology, University of Fribourg, Fribourg, Switzerland.
Mehdi ZouiouichLaboratoire de Physique de l'École Normale Supérieure, Université PSL, CNRS, Sorbonne Université, Université de Paris, Paris, France.
Francesca GiordanoInstitute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Universite Paris-Saclay, Gif-sur-Yvette cedex, France.
Stefano VanniDepartment of Biology, University of Fribourg, Fribourg, Switzerland.
Thomas J MeliaDepartment of Cell Biology, Yale University School of Medicine, New Haven, CT, USA.
Abdou Rachid ThiamLaboratoire de Physique de l'École Normale Supérieure, Université PSL, CNRS, Sorbonne Université, Université de Paris, Paris, France. abdou-rachid.thiam@ens.fr.ORCID http://orcid.org/0000-0001-7488-4724

Funding

Regulation of Autophagosome Membrane Dynamics by the AtgB Family of ProteinsR01GM100930 · NIGMS · YALE UNIVERSITY · PI MELIA, THOMAS JAMES · 2013 to 2024
$5.4M
ATG2 mediated lipid transport is essential for lipid droplet homeostasisF31DK136246 · NIDDK · YALE UNIVERSITY · PI KORFHAGE, JUSTIN · 2023 to 2025
$146k
NIDDK NIH HHS F31 DK136246NIGMS NIH HHS R01 GM100930
6 · The paper itself

Abstract

Lipid droplet (LD) growth mechanisms and the roles of LD-associated lipid transfer proteins remain poorly understood. Here we show that the autophagy lipid transfer protein ATG2A has an anabolic role and promotes LD expansion by transferring diacylglycerol (DAG), triacylglycerol (TAG) and phosphatidic acid, from the endoplasmic reticulum to LDs. In ATG2A deficiency, synthesized lipids are incorporated inefficiently into LDs and assemble new LDs. In addition, DAG O-acyltransferase 2 (DGAT2), which synthesizes TAG and expands LD, fails to relocate to LDs. In vitro, DAG recruits DGAT2 to LDs. These findings support the idea that ATG2A-mediated DAG transfer recruits DGAT2 to LDs, promoting LD expansion. ATG2A alone promotes LD growth by transferring TAG and DAG, but its effectiveness in LD expansion is reduced when DGAT2 is inhibited. This synergistic action with DGAT2 prevents the buildup of nonmembrane lipids within the endoplasmic reticulum and favors TAG synthesis on the LD surface.

Indexed as

Autophagy-Related ProteinsDiacylglycerol O-AcyltransferaseDiglyceridesLipid DropletsEndoplasmic ReticulumGene Knockout TechniquesHeLa CellsHumansMolecular Dynamics SimulationPhosphatidic AcidsTriglyceridesATG2A protein, humanAutophagy-Related ProteinsDGAT2 protein, humanDiacylglycerol O-AcyltransferaseDiglyceridesPhosphatidic AcidsTriglycerides

Identifiers

PMID41249819
PMCPMC12700830

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.