ArticleScientific reports2025
Droplet-based microfluidics platform for investigation of protoplast development of three exemplary plant species.
Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
3 citing papers in PubMed.
- A Scalable Droplet Microfluidic Platform That Overcomes Large-Cell Constraints for Long-Term Plant Cell Culture and Embryogenesis Analysis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Organ-on-a-Chip and Microfluidic Plant Cell Culture Systems: The Next Frontier for Controlled Secondary Metabolite Production and Real-Time Metabolomic Monitoring.Plants (Basel, Switzerland) · 2026Review
- Aggregates-on-chip: a microfluidic device for crowding plant cells in culture.Frontiers in plant science · 2026Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microfluidic technologies offer powerful tools for miniaturized and highly controlled biological experiments, yet their application in plant research remains underexploited. In this study, we present a droplet-based microfluidic platform tailored for the encapsulation and cultivation of plant protoplasts, enabling long-term observation of cell development at nearly single-cell resolution. Protoplasts isolated from leaves of Nicotiana tabacum, Brassica juncea, and Kalanchoe daigremontiana were used to evaluate the platform's suitability across diverse plant species. Our results demonstrate species-dependent responses to microfluidic cultivation, with tobacco protoplasts showing the highest viability. The system permits dynamic tracking of cell fate within individual droplets and supports the quantification of stochastic and concentration-dependent responses to chemical stimuli. Using tobacco protoplasts, we further investigated the effect of low concentrations of cytokinins (BAP) and auxins (NAA) for the early protoplast culture, up to the first division. Low concentrations (20-80 µg·L⁻¹) significantly enhanced cell survival and cell growth, while higher doses did not yield additional benefits. This work underscores the potential of droplet-based microfluidics as a high-resolution, low-volume platform for protoplast-based assays and dose-response screening, with applications across diverse plant biotechnology studies.
Indexed as
Identifiers
What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.