Evidence map›Paper›PMID 41254785›Full record

ArticleJournal of orthopaedic surgery and research2025

Huo Xue Tong Luo capsule epigenetically activates β-catenin, promotes Prx1-lineage cells proliferation and endochondral bone formation during fracture healing.

Yang Peng, Ding Wang, Qiangqiang Zhao, Liangliang Xu, Shuo Huang, Yamei Liu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of orthopaedic surgery and research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Yang Peng *Key Laboratory of Orthopaedics & Traumatology, Lingnan Medical Research Center, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, PR China.
Ding Wang *The Eighth Clinical Medical College of Guangzhou University of Chinese Medicine, Foshan Hospital of Traditional Chinese Medicine, Foshan, PR China.
Qiangqiang ZhaoKey Laboratory of Orthopaedics & Traumatology, Lingnan Medical Research Center, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, PR China.
Liangliang XuKey Laboratory of Orthopaedics & Traumatology, Lingnan Medical Research Center, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, PR China. xull-2016@gzucm.edu.cn.
Shuo HuangState Key Laboratory of Trauma, Burns and Combined Injury, Department of Wound Repair and Rehabilitation, Trauma Center, Research Institute of Surgery, Daping Hospital, Army Medical University, Chongqing, PR China. huangshuowork@foxmail.com.
Yamei LiuSchool of Basic Medical Science, Guangzhou University of Chinese Medicine, Guangzhou, PR China. gzhlym@gzucm.edu.cn.

Funding

National Natural Science Foundation of China 82172445
6 · The paper itself

Abstract

backgroundIn recent years, traditional Chinese medicine (TCM) has been noted to possess unique advantages in fracture treatment. In our previous studies, we have demonstrated that Huo Xue Tong Luo capsule (HXTL) promotes osteogenesis and inhibits osteoporosis. However, the effect of the HXTL on fracture healing has not been studied.

methodsMouse bone marrow mesenchymal stem cells (MSCs) were isolated and cultured. Western blotting, quantitative RT-PCR, immunohistochemical staining, immunofluorescence staining, and RNA-seq analysis were applied to detect the levels of genes or proteins. The mouse femoral fracture model was used to investigate the effect of HXTL on fracture healing.

resultsIn this study, our in vitro experiments confirmed that HXTL could enhance the proliferation and osteogenic differentiation of MSCs in a dose-dependent manner. Then, we established femur fracture healing model using the Prx1-CreERT

conclusionTaken together, our results showed that HXTL epigenetically activated β-catenin, promoted Prx1-lineage cells proliferation and endochondral bone formation during fracture healing, which provides insights into the potential therapeutic application of HXTL in bone regeneration and fracture healing.

Indexed as

beta CateninCell LineageCell ProliferationDrugs, Chinese HerbalEpigenesis, GeneticFracture HealingHomeodomain ProteinsOsteogenesisAnimalsCell DifferentiationCells, CulturedFemoral FracturesMaleMesenchymal Stem CellsMiceMice, Inbred C57BLbeta CateninCTNNB1 protein, mouseDrugs, Chinese HerbalHomeodomain ProteinsPrrx1 protein, mouseFracture healingHuo xue tong luo capsuleLineage tracingMesenchymal stem cellsPrx1

Identifiers

PMID41254785
PMCPMC12625412

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.