Evidence map›Paper›PMID 41255148›Full record

ArticleAngewandte Chemie (International ed. in English)2026

Boosting Reversible Photocontrol of a Photoxenase by an Engineered Conformational Shift.

Sabrina Mandl, Janet Sánchez, Miquel Estévez, Astrid Bruckmann, Caroline Hiefinger, Sílvia Osuna, Andrea Hupfeld

Abstract read
In one paragraph

Article in Angewandte Chemie (International ed. in English), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Sabrina MandlInstitute of Biophysics and Physical Biochemistry and Regensburg Center for Biochemistry, University of Regensburg, Universitätsstraße 31, D-93053, Regensburg, Germany.ORCID 0009-0005-2149-0656
Janet SánchezInstitut de Química Computacional i Catàlisi and Departament de Química, Universitat de Girona, c/Maria Aurèlia Capmany 69, Girona, 17003, Spain.ORCID 0009-0003-5258-4511
Miquel EstévezInstitut de Química Computacional i Catàlisi and Departament de Química, Universitat de Girona, c/Maria Aurèlia Capmany 69, Girona, 17003, Spain.ORCID 0000-0002-8576-8777
Astrid BruckmannInstitute of Biochemistry, Genetics and Microbiology, University of Regensburg, Universitatsstrasse 31, D-93053, Regensburg, Germany.
Caroline HiefingerInstitute of Biophysics and Physical Biochemistry and Regensburg Center for Biochemistry, University of Regensburg, Universitätsstraße 31, D-93053, Regensburg, Germany.ORCID 0009-0000-4587-9248
Sílvia OsunaInstitut de Química Computacional i Catàlisi and Departament de Química, Universitat de Girona, c/Maria Aurèlia Capmany 69, Girona, 17003, Spain.ORCID 0000-0003-3657-6469
Andrea HupfeldInstitute of Biophysics and Physical Biochemistry and Regensburg Center for Biochemistry, University of Regensburg, Universitätsstraße 31, D-93053, Regensburg, Germany.ORCID 0000-0002-4341-1593

Funding

Deutsche Forschungsgemeinschaft STE 891/12-2European Research CouncilEuropean Union's Horizon 2020 Research and Innovation Program ERC-2015-StG-679001European Union's Horizon 2020 Research and Innovation Program ERC-2022-CoG-101088032European Union's Horizon 2020 Research and Innovation Program ERC-2022-POC-101112805European Union's Horizon 2020 Research and Innovation Program ERC-2023-POC-101158166Generalitat de Catalunya TCBioSys (SGR 2021 0487)Human Frontier Science Program RGP0054/2020Spanish MICIN PDC2022-133950-I00Spanish MICIN PID2021-129034NB-I00Spanish MINECO PRE2022-105114
6 · The paper itself

Abstract

Our study successfully explores strategies to effectively improve the photocontrol efficiency of light-sensitive enzymes, dubbed photoxenases, with photoswitchable unnatural amino acids (UAAs). The engineering of photoxenases is a versatile method for the reversible photocontrol in various applications. To boost the photocontrol of an established allosteric and heterodimeric photoxenase based on imidazole glycerol phosphate synthase, we turned from an ineffective tuning of the UAA photochemistry to a semi-rational enzyme design. Remarkably, mutations at the catalytically important heterodimer interface increased the light-regulation factor (LRF) for the k

Indexed as

AminohydrolasesProtein EngineeringAmino AcidsKineticsLightPhotochemical ProcessesProtein ConformationAmino AcidsAminohydrolasesimidazole glycerol phosphate synthaseCorrelation‐based methodsEnzyme catalysisMolecular dynamicsPhotocontrolUnnatural amino acids

Identifiers

PMID41255148
PMCPMC12828452

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.