ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2026
A Hydrodynamic Bioreactor for High-Yield Production of Extracellular Vesicles from Stem Cell Spheroids with Defined Cargo Profiling.
Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
5 citing papers in PubMed.
- Regulatory Challenges and Opportunities for Cell-Derived Extracellular Vesicles in Pharmaceutical Development: A European and Global Perspective.Journal of extracellular vesicles · 2026Review
- Extracellular Vesicles as Enabling Biomarkers for New Approach Methodologies to Support the US Food and Drug Administration Modernization Act 3.0.Journal of extracellular vesicles · 2026Review
- Plant-Derived Nanocarriers for Drug Delivery: A Unified Framework Integrating Extracellular Vesicles, Engineered Phytocarriers, Hybrid Platforms, and Bioinspired Systems.Plants (Basel, Switzerland) · 2026Review
- A Hydrodynamic Bioreactor for High-Yield Production of Extracellular Vesicles from Stem Cell Spheroids with Defined Cargo Profiling.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Organoid-Derived Extracellular Vesicles: From Biogenesis and Cargo Mechanisms Toward Therapeutic Applications.International journal of nanomedicine · 2026Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
Abstract
Extracellular vesicles (EVs) have become pivotal in clinical therapeutics, although no consensus exists on optimal production methods. Here, an innovative approach and associated device are introduced that employ hydrodynamic stress to form and stimulate stem cell spheroids. The method involves culturing cellular material in vessels equipped with internal obstacles, subjected to rotational motion at varying speeds and in alternating reversing modes. It achieves EV yields 10 to 20 times greater than those obtained with traditional static culture methods, offering a robust and high-output method compatible with large-scale EV manufacturing. This approach not only streamlines spheroid formation and subsequent EV release into a single, integrated process but also ensures the generation of EVs with enhanced biological activity and a distinctive protein cargo signature. Notably, hydrodynamic stimulation enriched EVs with ectosome- and mitochondria-associated proteins, suggesting distinct biogenesis pathways. Additionally, these EVs demonstrated superior functionality in promoting wound healing, angiogenesis, and anti-inflammatory responses, underscoring their therapeutic potential.
Indexed as
Identifiers
What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.