Evidence mapPaperPMID 41255264Full record

ReviewAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2026

Methodologies for Sample Multiplexing and Computational Deconvolution in Single-Cell Sequencing.

Yufei Gao, Weiwei Yin, Wei Hu, Wei Chen

Abstract readReview
In one paragraph

Review in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Yufei GaoDepartment of Cardiology and Department of Cell Biology of the Second Affiliated Hospital, Liangzhu Laboratory, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310012, China.ORCID https://orcid.org/0009-0002-3446-309X
Weiwei YinKey Laboratory for Biomedical Engineering of the Ministry of Education, College of Biomedical Engineering and Instrument Science, Zhejiang University, Hangzhou, Zhejiang, 310012, China.ORCID https://orcid.org/0000-0001-9142-6186
Wei HuKidney Disease Center, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310012, China.ORCID https://orcid.org/0000-0003-3068-6333
Wei ChenDepartment of Cardiology and Department of Cell Biology of the Second Affiliated Hospital, Liangzhu Laboratory, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310012, China.ORCID https://orcid.org/0000-0001-5366-7253

Funding

National Natural Science Foundation of China 92269101National Natural Science Foundation of China 92359303National Natural Science Foundation of China T2394510National Natural Science Foundation of China T2394511National Science and Technology Major Project 2023ZD0501300Natural Science Foundation of Zhejiang Province LY23A020002
6 · The paper itself

Abstract

Single-cell sequencing is revolutionizing biological research by enabling unprecedented cellular resolution, yet traditional multi-sample experiments are often constrained by high costs and batch effects. Sample multiplexing offers a critical solution by uniquely tagging individual cells from diverse samples for pooled sequencing, thereby dramatically boosting throughput and improving data reliability by minimizing technical variability. This review provides a comprehensive and integrated perspective on the rapidly evolving field of single-cell multiplexing. Major experimental strategies and the critical computational algorithms required for accurate sample deconvolution are surveyed, highlighting the crucial link between experimental design and computational accuracy. Furthermore, the diverse applications of these technologies in large-scale clinical cohorts, multi-omics integration, developmental biology, and high-throughput drug screening are summarized. This review serves as an essential guide for researchers, empowering them to select the most appropriate methods to accelerate discoveries in disease mechanisms, therapeutic responses, and developmental biology.

Indexed as

Computational BiologyHigh-Throughput Nucleotide SequencingSingle-Cell AnalysisAlgorithmsHumanscomputational deconvolutionsample multiplexingsingle‐cell sequencing

Identifiers

PMID41255264
PMCPMC12806368

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.