ArticleJournal of ovarian research2025
Effects of physiological oxygen tension on human cumulus-oocyte-complex metabolism during in vitro maturation: an exploratory study.
Article in Journal of ovarian research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
4 citing papers in PubMed.
- Impacts of Oxygen Tension on Developmental Competence of Preimplantation Embryos.Biomolecules · 2026Review
- Metabolic Comparison of Mature and Immature Bovine Cumulus-Oocyte Complexes with Standardization of Bioenergetic Assessment.Biomolecules · 2026Article
- Advances, Mechanisms, and Clinical Perspectives for the In Vitro Maturation of Human Oocytes.International journal of molecular sciences · 2025Review
- The First Successful Capacitation In Vitro Maturation of Oocyte in Indonesia: A Promising Strategy to Enhance IVF Accessibility for Infertile Couples.Journal of reproduction & infertilityArticle
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
In vitro oocyte maturation (IVM) is an internationally recognized, patient-friendly assisted reproductive technology, particularly valuable for women with polycystic ovary syndrome (PCOS). Biphasic IVM protocols, incorporating a pre-IVM step to promote cytoplasmic maturation under meiotic arrest, outperform standard IVM yet remain inferior to conventional methods involving controlled ovarian stimulation. Considering suboptimal culture conditions, such as supraphysiological oxygen, might be responsible for lower IVM oocyte competence, in this study, we investigated energy metabolism in human sibling cumulus-oocyte-complexes (COCs) through culturing them under different oxygen concentrations during pre-IVM. COCs (n = 239) from follicles < 10 mm, collected in non-PCOS hyper-responder research oocyte donors (n = 21) with minimal (or no) stimulation prior to oocyte retrieval, were allocated for the study. COCs per donor were randomly cultured in both 20% (atmospheric) and 5% (physiological) oxygen. After 24-hours pre-IVM culture, the samples were processed to (i) assess COC metabolic function with Seahorse ATP Production Rate Assay and MitoStress Test, (ii) measure COC ATP concentrations, (iii) analyze spent medium (glucose, lactate, amino acids), and (iv) evaluate oocyte mitochondrial and antioxidative functions (CellROX, JC-1, and mBCl stainings). COCs cultured under 5% oxygen contained higher ATP despite lower mitochondrial respiration, accompanied by steady glucose uptake and increased lactate production, while mitochondrial potential and antioxidative markers remained unchanged. These findings suggest reliance on an alternative energy substrate, such as fatty acids, under low oxygen conditions. By demonstrating improved metabolic efficiency in human COCs under physiological oxygen, this study highlights the need to re-evaluate IVM culture conditions to better mimic the in vivo environment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.