Identification and mechanism of key genes in inflammatory response of chronic otitis media in rats.

Oulai Li, Yan Lin, Rui Wang, Jiazhi Yin, Lijuan Zhou, Yong Tang

Abstract read

In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Oulai Li *Department of Otolaryngology, Head and Neck Surgery, The First Affiliated Hospital of Kunming Medical University, Kunming, 650000, China.

Yan Lin *Department of Otolaryngology, Head and Neck Surgery, The First Affiliated Hospital of Kunming Medical University, Kunming, 650000, China.

Rui WangDepartment of Otolaryngology, Head and Neck Surgery, The First Affiliated Hospital of Kunming Medical University, Kunming, 650000, China.

Jiazhi YinDepartment of Otolaryngology, Head and Neck Surgery, The First Affiliated Hospital of Kunming Medical University, Kunming, 650000, China.

Lijuan ZhouDepartment of Otolaryngology Head and Neck Surgery, The Affiliated Children's Hospital of Kunming Medical University, Kunming, 650000, China.

Yong TangDepartment of Otolaryngology, Head and Neck Surgery, The First Affiliated Hospital of Kunming Medical University, Kunming, 650000, China. yongtang0929@sina.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Chronic suppurative otitis media (CSOM) affects 330 million people globally; however, biomarkers for its inflammatory response (IR) remain underinvestigated. This study aimed to identify key genes in IR and explore the mechanisms of CSOM. Following the CSOM model construction, RNA sequencing was performed on rat samples and IR-related genes were extracted from public databases. The key genes were identified using the support vector machine recursive feature elimination and least absolute shrinkage and selection operator algorithms, and the diagnostic performance was assessed using receiver operating characteristic curves. Nucleoside diphosphate kinase (NDK) protein expression was specifically validated via immunohistochemical analysis. A nomogram was created to predict the incidence of CSOM, and functional enrichment, drug prediction, and molecular docking analyses were conducted. The results indicated that three key genes (Has2, Clec5a, and Il6) were markedly upregulated in CSOM, validated by immunohistochemical staining and quantitative polymerase chain reaction analysis. These findings provide insights into potential CSOM therapies.

Indexed as

Gene Expression RegulationOtitis Media, SuppurativePseudomonas InfectionsAnimalsChronic DiseaseDisease Models, AnimalHyaluronan SynthasesImmunohistochemistryInterleukin-6Lectins, C-TypeMaleNucleoside-Diphosphate KinasePseudomonas aeruginosaRatsRats, Sprague-DawleyHas2 protein, ratHyaluronan SynthasesIl6 protein, ratInterleukin-6Lectins, C-TypeNucleoside-Diphosphate KinaseChronic suppurative otitis mediaImmunohistochemical analysisInflammatory responseKey genesMolecular dockingTranscriptome sequencing

Identifiers

PMID41258032

DOI10.1038/s41598-025-24626-z

PMCPMC12630814

What Socratic holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.