Evidence mapPaperPMID 41258511Full record

ArticleScientific reports2025

circRNA-associated-ceRNA networks in patients with traumatic tracheal stenosis.

Shujuan Qin, Wentao Li, Jinmei Wei, Yuhui Wei, Yinning Zhou, Pingping Huang, Sen Tan, Guangnan Liu

Abstract read
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Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

8 authors.

Shujuan Qin *Department of Respiratory and Critical Care Medicine, the Second Affiliated Hospital of Guangxi Medical University, Nanning, China.
Wentao Li *Department of Respiratory and Critical Care Medicine, the Second Affiliated Hospital of Guangxi Medical University, Nanning, China.
Jinmei WeiDepartment of Respiratory and Critical Care Medicine, the Second Affiliated Hospital of Guangxi Medical University, Nanning, China.
Yuhui WeiDepartment of Respiratory and Critical Care Medicine, the Second Affiliated Hospital of Guangxi Medical University, Nanning, China.
Yinning ZhouDepartment of Respiratory and Critical Care Medicine, the Second Affiliated Hospital of Guangxi Medical University, Nanning, China.
Pingping HuangDepartment of Respiratory and Critical Care Medicine, the Second Affiliated Hospital of Guangxi Medical University, Nanning, China.
Sen TanDepartment of Respiratory and Critical Care Medicine, the Second Affiliated Hospital of Guangxi Medical University, Nanning, China.
Guangnan LiuDepartment of Respiratory and Critical Care Medicine, the Second Affiliated Hospital of Guangxi Medical University, Nanning, China. gnliu63@hotmail.com.

Funding

Joint Project on Regional High-Incidence Diseases Research of Guangxi Natural Science Foundation under Grant 2024GXNSFAA010082National Natural Science Foundation of China 82360026
6 · The paper itself

Abstract

Traumatic tracheal stenosis (TTS) is one of the most commonly seen iatrogenic airway injuries caused by tracheal intubation or tracheotomy, which can harm the lives of patients in severe cases. Circular RNA (circRNA) is related to a variety of human diseases. However, the role that circRNAs are likely to play in traumatic tracheal stenosis is not known to a large extent till now. This research is designed to identify potential biomarkers and learn more about the mechanism of TTS through the establishment of a circRNA-associated ceRNA network. By carrying out RNA sequencing (RNA-seq), the expression profiles of circRNAs and mRNAs were explored from four TTS patients and four normal control cases. To investigate the differentially expressed circRNAs and mRNAs, this research performed both Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses. A circRNA-miRNA-mRNA network was predicted and established using bioinformatics tools including Mireap, Miranda, TargetScan, the circBase database, and miRTarBase. And then verified by utilizing RT-qPCR assays from six TTS patients and six normal control cases. In clinical tissue samples of the TTS group and the control group, 603 circRNAs and 1950 mRNAs were differentially expressed. According to GO and KEGG analyses, the differentially expressed circRNAs mainly participated in lysine degradation, platelet activation, and the VEGF/HIF-1 signaling pathway, whereas the differentially expressed mRNAs mainly participated in cytokine-cytokine receptor interaction, ECM-receptor interaction, neutrophil extracellular trap formation, and the PI3K-Akt signaling pathway. By utilizing 17 circRNAs, 7 miRNAs, and 9 mRNAs, a competing endogenous RNA (ceRNA) network was set up. Using RT-qPCR assay, It was confirmed that S100A2 and circ_030284 were upregulated, while miR-1207-5p was downregulated in TTS patients compared to normal controls, consistent with the gene chip outcomes. The research presents a comprehensive circRNA-associated ceRNA network in patients with TTS, which provides novel insights into TTS’s underlying pathogenesis.

Indexed as

Gene Regulatory NetworksRNA, CircularTracheal StenosisAdultBiomarkersCase-Control StudiesComputational BiologyFemaleGene Expression ProfilingGene Expression RegulationGene OntologyHumansMaleMicroRNAsMiddle AgedRNA, Competitive EndogenousBiomarkersMicroRNAsRNA, CircularRNA, Competitive EndogenousRNA, MessengerGene expression profilingRNA, circularRNA, messengerSequence analysis, RNATracheal stenosis

Identifiers

PMID41258511
PMCPMC12630782

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.