Evidence mapPaperPMID 41259533Full record

ArticleScience advances2025

Targeting cancer-associated fibroblasts for real-time intraoperative tumor identification with a spray-on fluorescent probe.

Riley J Deutsch-Williams, Yuxuan Xie, Zachary Rabinowitz, Marie Goemans, Pratyaksha Wirapati, Claudio Vinegoni, Jonathan Ct Carlson, Mikael Pittet, Ralph Weissleder

Abstract read
In one paragraph

Article in Science advances, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Erasable FAP Targeted Spray-On Probe for Fluorescence-Guided Surgery.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026
    Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Riley J Deutsch-WilliamsCenter for Systems Biology, Massachusetts General Hospital, 185 Cambridge St., CPZN 5206, Boston, MA 02114, USA.ORCID 0000-0001-8483-5029
Yuxuan XieDepartment of Pathology and Immunology and Center for Translational Oncohaematology Research, University of Geneva, Geneva, Switzerland.ORCID 0009-0008-0640-2269
Zachary RabinowitzCenter for Systems Biology, Massachusetts General Hospital, 185 Cambridge St., CPZN 5206, Boston, MA 02114, USA.ORCID 0009-0002-1250-5164
Marie GoemansCenter for Systems Biology, Massachusetts General Hospital, 185 Cambridge St., CPZN 5206, Boston, MA 02114, USA.ORCID 0009-0008-9025-1888
Pratyaksha WirapatiAGORA Cancer Research Center and Swiss Cancer Center Leman, Lausanne, Switzerland.ORCID 0000-0002-0327-4686
Claudio VinegoniCenter for Systems Biology, Massachusetts General Hospital, 185 Cambridge St., CPZN 5206, Boston, MA 02114, USA.ORCID 0000-0002-9818-3639
Jonathan Ct CarlsonCenter for Systems Biology, Massachusetts General Hospital, 185 Cambridge St., CPZN 5206, Boston, MA 02114, USA.ORCID 0000-0003-4139-9057
Mikael PittetDepartment of Pathology and Immunology and Center for Translational Oncohaematology Research, University of Geneva, Geneva, Switzerland.ORCID 0000-0002-2060-4691
Ralph WeisslederCenter for Systems Biology, Massachusetts General Hospital, 185 Cambridge St., CPZN 5206, Boston, MA 02114, USA.ORCID 0000-0003-0828-4143

Funding

FAST-FNA immune cell profiling in HNSCCR01CA257623 · NCI · MASSACHUSETTS GENERAL HOSPITAL · 2022 to 2025
$2.0M
TRAINING GRANT IN MOLECULAR IMAGING RESEARCHT32CA079443 · MASSACHUSETTS GENERAL HOSPITAL · 2000 to 2025
$1.6M
Temporal analysis of the GBM tumor microenvironment during myeloid cell activating therapyR01CA281735 · MASSACHUSETTS GENERAL HOSPITAL · 2025 to 2025
$584k
Bioorthogonal probe development for highly parallel in vivo imagingR33CA277820 · MASSACHUSETTS GENERAL HOSPITAL · 2025 to 2025
$416k
NCI NIH HHS R01 CA257623NCI NIH HHS R01 CA281735NCI NIH HHS R33 CA277820NCI NIH HHS T32 CA079443
6 · The paper itself

Abstract

Surgical tumor resection is often the only curative option for the nearly 20 million newly diagnosed patients with cancer every year. Fluorescence-guided surgery techniques are being developed in an effort to improve margin detection and surgical resection outcomes, with several systemically administered imaging agents having gained clinical approval. However, it has been challenging to overcome limited margin contrast with current approaches and to navigate procedural complexities of intravenous contrast delivery. We hypothesized that "spray-on probes" with specificity for fibroblast activation protein alpha in peritumoral fibroblasts could improve fluorescence-guided surgery, detect smaller tumors, improve imaging accuracy, and reduce the amount of times a patient is hospitalized. We show that this strategy increases achievable tumor margin contrast by 5- to 10-fold and detects even microscopic cancer deposits. These improvements have the potential to transform patient outcomes by enabling more accurate cancer surgeries, reducing the number of follow-up surgeries, and leading to personalized treatment plans.

Indexed as

Cancer-Associated FibroblastsFluorescent DyesNeoplasmsSurgery, Computer-AssistedAnimalsCell Line, TumorEndopeptidasesFibroblast Activation Protein AlphaHumansMembrane ProteinsMiceOptical ImagingEndopeptidasesFibroblast Activation Protein AlphaFluorescent DyesMembrane Proteins

Identifiers

PMID41259533
PMCPMC12629203

What Socratic holds

Textmetadata
LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.