Evidence map›Paper›PMID 41263277›Full record

ArticleVeterinary medicine and science2025

Regulation of Milk Production by the MAPK/ERK Pathway in Water Buffalo (Bubalus bubalis): Genomic and Molecular Insights.

Saima Naz, Urwah Ishaque, Ahmad Manan Mustafa Chatha, Babar Maqbool, Qudrat Ullah, Muhammad Farooq, Shabana Naz, Naseer Khan Momand, Ibrahim A Alhidary

Abstract read
In one paragraph

Article in Veterinary medicine and science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Saima NazDepartment of Zoology, Government Sadiq College Women University, Bahawalpur, Punjab, Pakistan.
Urwah IshaqueDepartment of Zoology, Government Sadiq College Women University, Bahawalpur, Punjab, Pakistan.
Ahmad Manan Mustafa ChathaDepartment of Entomology, Faculty of Agriculture and Environment, The Islamia University of Bahawalpur, Bahawalpur, Punjab, Pakistan.
Babar MaqboolDepartment of Veterinary Science, Faculty of Animal Husbandry and Veterinary Sciences, The University of Agriculture, Dera Ismail Khan, Pakistan.ORCID https://orcid.org/0000-0001-8197-3119
Qudrat UllahDepartment of Theriogenology, Cholistan University of Veterinary and Animal Sciences, Bahawalpur, Pakistan.
Muhammad FarooqDepartment of soil plant and Food science university of Bari Aldo Moro, Bari, Italy.
Shabana NazDepartment of Zoology, Government College Univeristy, Faisalabad, Pakistan.
Naseer Khan MomandAnimal Science, Nangarhar University, Jalalabad, Afghanistan.ORCID https://orcid.org/0009-0004-8478-091X
Ibrahim A AlhidaryDepartment of Animal Production, College of Food and Agriculture Science, King Saud Univeristy, Riyadh, Saudi Arabia.

Funding

King Saud University ORF-2025-833
6 · The paper itself

Abstract

The MAPK/ERK pathway plays a critical role in the regulation of milk production by controlling cellular processes such as proliferation, differentiation and survival, which are essential for lactogenesis and mammary gland function. Bubalus bubalis (Water buffalo), known for its unique physiological and ecological characteristics, serves as an ideal model to explore the evolutionary and molecular roles of MAPK/ERK pathway genes. This study presents the first comprehensive computational analysis of MAPK/ERK genes in B. bubalis, identifying 21 key genes involved in the pathway. Phylogenetic analysis clustered these genes into 13 distinct clades, such as MST1, GRB2, RAS, ETS1, JUN and FOS, and revealed close evolutionary relationships with Bos taurus and Camelus bactrianus. Structural characterization identified 10 conserved motifs, including essential domains like protein kinase, ETS and RAS, reflecting their functional significance. Gene structure analysis revealed substantial variation in exon-intron patterns, while synteny analysis confirmed collinearity with human orthologs, indicating genomic conservation. Physicochemical analysis highlighted a broad range of molecular weights and isoelectric points, with most proteins classified as hydrophilic and thermostable. Gene duplication and selection analyses revealed seven segmentally duplicated gene pairs, with the JUN-ETS1 and DUSP6-MST1 pairs showing evidence of positive selection, suggesting functional divergence. These findings establish a foundational understanding of MAPK/ERK pathway genes in B. bubalis and provide valuable insights into potential targets for genetic improvement, selective breeding and sustainable dairy management strategies aimed at enhancing milk production and quality.

Indexed as

BuffaloesLactationMAP Kinase Signaling SystemMilkAnimalsFemaleGenomicsPhylogenygenome characterizationlactation regulationMAPK/ERK pathway genesmilk productionphylogenetic analysistranscriptional regulation

Identifiers

PMID41263277
PMCPMC12631544

What Socratic holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.