Evidence mapPaperPMID 41263995Full record

ArticleNaunyn-Schmiedeberg's archives of pharmacology2026

Myricetin ameliorates letrozole-induced PCOS in female rats-a mechanistic approach through in silico, in vitro, and in vivo study via P13/AKT and NF-κB pathway crosstalk.

Samia Gul Niazi, Uzma Saleem, Liaqat Hussain, Ammara Saleem

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Article in Naunyn-Schmiedeberg's archives of pharmacology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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0cells of the map it votes in
1citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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1 citing paper in PubMed.

  1. Review
4 · The record

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5 · Who and what money

Authors and funding

4 authors.

Samia Gul NiaziFaculty of Pharmaceutical Sciences, Government College University Faisalabad, Faisalabad, 3800, Pakistan.
Uzma SaleemFaculty of Pharmaceutical Sciences, Government College University Faisalabad, Faisalabad, 3800, Pakistan. uzma95@gmail.com.
Liaqat HussainFaculty of Pharmaceutical Sciences, Government College University Faisalabad, Faisalabad, 3800, Pakistan.
Ammara SaleemFaculty of Pharmaceutical Sciences, Government College University Faisalabad, Faisalabad, 3800, Pakistan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Polycystic ovarian syndrome (PCOS) is one of the leading causes of abnormalities in young female patients. Myricetin is flavonoid which has multiple pharmacological effects like anti-inflammatory, antidiabetic, and anticancer. This study determined the effect of myricetin in the treatment of PCOS. For in silico studies, network pharmacology and molecular docking and simulation were done through multiple software tools. In vitro evaluation was done through antioxidant assays (DPPH and ABTS). Female Sprague Dawley rats were used in six groups. Letrozole (1 mg/kg) in 0.5% CMC was used to induce PCOS for 21 days, while CMC was the only treatment given to the normal control. Metformin (20 mg/kg) or myricetin (75, 150, and 300 mg/kg) was administered for 15 days. On day 37, ovarian tissues were homogenized for antioxidant tests and RT-qPCR gene expression analysis, reproductive organs were gathered for histology, and blood was processed for ELISA and LFTs. Network pharmacology shows significant effects of myricetin within the body; however, molecular docking and simulation studies show that Protein kinase B (AKT) has a greater binding capacity with myricetin. It also shows maximum antioxidant activity through antioxidant assays. Myricetin's safety was validated by biochemical, oxidative stress, and histopathological analyses. It enhanced follicular structures and significantly reduced body weight and ovarian cysts. By lowering testosterone (0.265 ± 0.021 mIU/mL vs. 0.735 ± 0.14), LH (0.518 ± 0.009 vs. 2.005 ± 0.027), insulin (11.56 ± 0.40 vs. 29.21 ± 0.59), and corticosterone (51.8 ± 0.79 vs. 115.5 ± 2.9), while increasing FSH (0.87 ± 0.30 mIU/mL vs. 0.36 ± 0.04), myricetin restored antioxidant status. Gene expression analysis shows higher level of PCOS-related genes. Given that these effects were similar to those of metformin, myricetin appears to be a promising treatment option for PCOS.

Indexed as

FlavonoidsNF-kappa BPolycystic Ovary SyndromeProto-Oncogene Proteins c-aktAnimalsAntioxidantsComputer SimulationDisease Models, AnimalFemaleLetrozoleMolecular Docking SimulationOvaryOxidative StressRatsRats, Sprague-DawleySignal TransductionAntioxidantsFlavonoidsLetrozolemyricetinNF-kappa BProto-Oncogene Proteins c-aktAnti-inflammatoryCystic folliclesGene expressionMolecular dockingNetwork pharmacologyOxidative stressPolycystic ovaries

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.