Evidence mapPaperPMID 41268259Full record

ArticleAmerican journal of translational research2025

Silencing CDH17 reverses cisplatin resistance in gastric cancer cells by regulating the Warburg effect mediated by the Wnt/β-catenin pathway.

Xia Tian, Zheng Han, Ye Yu, Jie Tan, Qingxi Zhu, Wei Chen, Shasha Huang, Xiaoli Chen, Meng Liu

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Article in American journal of translational research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

9 authors.

Xia TianDepartment of Gastroenterology, Wuhan Third Hospital (Tongren Hospital of Wuhan University) Wuhan 430060, Hubei, China.
Zheng HanDepartment of Gastroenterology, Wuhan Third Hospital (Tongren Hospital of Wuhan University) Wuhan 430060, Hubei, China.
Ye YuSchool of Medicine, Jianghan University Wuhan 430056, Hubei, China.
Jie TanDepartment of Gastroenterology, Wuhan Third Hospital (Tongren Hospital of Wuhan University) Wuhan 430060, Hubei, China.
Qingxi ZhuDepartment of Gastroenterology, Wuhan Third Hospital (Tongren Hospital of Wuhan University) Wuhan 430060, Hubei, China.
Wei ChenDepartment of Gastroenterology, Wuhan Third Hospital (Tongren Hospital of Wuhan University) Wuhan 430060, Hubei, China.
Shasha HuangDepartment of Gastroenterology, Wuhan Third Hospital (Tongren Hospital of Wuhan University) Wuhan 430060, Hubei, China.
Xiaoli ChenDepartment of Gastroenterology, Wuhan Third Hospital (Tongren Hospital of Wuhan University) Wuhan 430060, Hubei, China.
Meng LiuDepartment of Gastroenterology, Wuhan Third Hospital (Tongren Hospital of Wuhan University) Wuhan 430060, Hubei, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTo investigate the role of CDH17 in cisplatin (DDP) resistance in gastric cancer (GC) and to elucidate the potential molecular mechanisms.

methodsDDP-resistant GC cell lines were established, and CDH17 expression was silenced in these cells. Cell proliferation was assessed using the MTT assay. Cellular glycolytic activity was quantified, and apoptosis was assessed using flow cytometry. The expression of CDH17, β-catenin, key Warburg-related effector proteins, and Cleaved-caspase-3 was determined through molecular experiments. The Warburg effect inhibitor 2-DG and the Wnt/β-catenin signaling pathway agonist CP21R7 were applied to investigate the underlying molecular mechanism.

resultsCDH17 was significantly upregulated in the DDP-resistant GC cells. CDH17 knockdown increased the sensitivity of GC cells to DDP, suppressed the Warburg effect, and inhibited the activation of the Wnt/β-catenin signaling pathway. Treatment with 2-DG reduced the chemoresistance in resistant GC cells. However, CP21R7 treatment promoted the Warburg effect in the DDP-resistant GC cells and partially attenuated the effects of CDH17 silencing on both the Warburg effect and DDP sensitivity.

conclusionCDH17 silencing reversed DDP resistance in GC cells, primarily through inhibition of the Warburg effect mediated by the Wnt/β-catenin signaling pathway.

Indexed as

cadherin 17chemotherapy resistanceGastric cancerWarburg effectWnt/β-catenin signaling pathway

Identifiers

PMID41268259
PMCPMC12628240

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.