Evidence map›Paper›PMID 41269556›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Investigating Dysregulated X Chromosome Inactivation in Human B Cells and Plasmacytoid Dendritic Cells by RNA FISH.

Berenice Faz-Lopez, Sophie Allart, Julie Chaumeil, Jean-Charles Guéry

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Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Berenice Faz-LopezUniv Toulouse, INSERM, CNRS, Infinity - Toulouse Institute for Infectious and Inflammatory Diseases, Toulouse, France.
Sophie AllartUniv Toulouse, INSERM, CNRS, Infinity - Toulouse Institute for Infectious and Inflammatory Diseases, Toulouse, France.
Julie ChaumeilUniversité Paris Cité, CNRS, INSERM, Institut Cochin, Paris, France. julie.chaumeil@inserm.fr.
Jean-Charles GuéryUniv Toulouse, INSERM, CNRS, Infinity - Toulouse Institute for Infectious and Inflammatory Diseases, Toulouse, France. jean-charles.guery@inserm.fr.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

RNA fluorescent in situ hybridization (RNA FISH) has been widely used to monitor the allelic expression of primary transcripts and/or non-coding RNAs in individual cell nuclei. In particular, this method is a gold standard to establish which X-linked genes undergo or escape X chromosome inactivation in female mammalian cells by combining probes specific of the X-linked transcripts of interest with probe specific of the long-non-coding XIST RNA that coats the inactive X (Xi) territory. Here we describe the dual RNA FISH protocol we have developed to track (1) the coating of the Xi by the XIST RNA and (2) the expression of the X-linked TLR7 gene on the active and inactive X chromosomes, in human plasmacytoid dendritic cells (pDCs) and in female B lymphocytes induced to differentiate into plasmablasts or pre-Antibody Secreting Cells (pre-ASC) upon activation of either TLR7 or TLR9.

Indexed as

B-LymphocytesDendritic CellsIn Situ Hybridization, FluorescenceX Chromosome InactivationChromosomes, Human, XFemaleHumansRNA, Long NoncodingToll-Like Receptor 7RNA, Long NoncodingTLR7 protein, humanToll-Like Receptor 7XIST non-coding RNAB cellsHuman immune cellsPlasmablastsPlasmacytoid dendritic cellsPre-Antibody Secreting CellsRNA FISHTLR7X chromosome inactivationXIST RNA

Identifiers

PMID41269556

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.