Evidence map›Paper›PMID 41272432›Full record

ArticleBMC genomics2025

Single-cell analysis reveals cellular heterogeneity and molecular features during postnatal pig testis development.

Lulu Wang, Hong Ao, Huatao Liu, Qingyao Zhao, Fuping Ma, Jinyan Yang, Jiahao Wang, Xini Wang, Guohong Liu, Xinran Niu and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC genomics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Lulu WangNational Engineering Laboratory for Animal Breeding and MOA Key Laboratory of Animal Genetics and Breeding, Department of Animal Genetics and Breeding, China Agricultural University, Beijing, 100193, China.
Hong AoBeijing Institute of Animal Science and Veterinary Medicine, Chinese Academy of Agricultural Sciences, Beijing, 1000193, China.
Huatao LiuNational Engineering Laboratory for Animal Breeding and MOA Key Laboratory of Animal Genetics and Breeding, Department of Animal Genetics and Breeding, China Agricultural University, Beijing, 100193, China.
Qingyao ZhaoNational Engineering Laboratory for Animal Breeding and MOA Key Laboratory of Animal Genetics and Breeding, Department of Animal Genetics and Breeding, China Agricultural University, Beijing, 100193, China.
Fuping MaNational Engineering Laboratory for Animal Breeding and MOA Key Laboratory of Animal Genetics and Breeding, Department of Animal Genetics and Breeding, China Agricultural University, Beijing, 100193, China.
Jinyan YangNational Engineering Laboratory for Animal Breeding and MOA Key Laboratory of Animal Genetics and Breeding, Department of Animal Genetics and Breeding, China Agricultural University, Beijing, 100193, China.
Jiahao WangNational Engineering Laboratory for Animal Breeding and MOA Key Laboratory of Animal Genetics and Breeding, Department of Animal Genetics and Breeding, China Agricultural University, Beijing, 100193, China.
Xini WangNational Engineering Laboratory for Animal Breeding and MOA Key Laboratory of Animal Genetics and Breeding, Department of Animal Genetics and Breeding, China Agricultural University, Beijing, 100193, China.
Guohong LiuNational Engineering Laboratory for Animal Breeding and MOA Key Laboratory of Animal Genetics and Breeding, Department of Animal Genetics and Breeding, China Agricultural University, Beijing, 100193, China.
Xinran NiuNational Engineering Laboratory for Animal Breeding and MOA Key Laboratory of Animal Genetics and Breeding, Department of Animal Genetics and Breeding, China Agricultural University, Beijing, 100193, China.
Zhuoye ZhengNational Engineering Laboratory for Animal Breeding and MOA Key Laboratory of Animal Genetics and Breeding, Department of Animal Genetics and Breeding, China Agricultural University, Beijing, 100193, China.
Chuduan WangNational Engineering Laboratory for Animal Breeding and MOA Key Laboratory of Animal Genetics and Breeding, Department of Animal Genetics and Breeding, China Agricultural University, Beijing, 100193, China. cdwang@cau.edu.cn.
Kai XingNational Engineering Laboratory for Animal Breeding and MOA Key Laboratory of Animal Genetics and Breeding, Department of Animal Genetics and Breeding, China Agricultural University, Beijing, 100193, China. xk@cau.edu.cn.

Funding

National Key R&D Program of China 2023YFD1300400STI 2030-Major Projects 2023ZD04046
6 · The paper itself

Abstract

backgroundPostnatal testicular development involves the proliferation and differentiation of somatic and spermatogenic cells. The entire process is governed by spatial and temporal cues that coordinate spermatogenesis. Comparative analyses of gene expression and transcriptional regulation across multiple stages can provide insights into the complexity of developmental dynamics. Therefore, we systematically analyzed the cellular composition and molecular features of boar testes at different time points using single-cell RNA sequencing (scRNA-seq) to reveal their roles in testicular development.

resultsWe performed scRNA-seq on 115,248 testicular cells from eight pigs at 0, 2, 5, 24, and 48 months of age, identifying 10 distinct cell types, including three rare populations: pericytes, smooth muscle cells, and neutrophils. Trajectory analysis and RNA velocity modeling uncovered dynamic cell fate transitions during spermatogenesis. Furthermore, we identified testis cell-specific transcription factors and regulators. Notably, PRDX5 and LUZP2 exhibited developmental stage-specific expression after M5 and varied with the proportion of germ cells. Cross-species comparative analysis revealed the evolutionary conservation among testicular cell populations, especially endothelial and germ cells, and identified several conserved transcription factors such as SOX9, IRF8, and KDM5B, highlighting conserved regulatory mechanisms during testicular development.

conclusionsOur findings provide novel insights into the cellular dynamics of spermatogenesis and offer a valuable foundation for future research on the molecular mechanisms underlying testicular development in pigs and other mammals.

Indexed as

Single-Cell AnalysisTestisAnimalsGene Expression ProfilingGene Expression Regulation, DevelopmentalMaleRNA-SeqSequence Analysis, RNASpermatogenesisSwineDevelopmental stagesHeterogeneityPigSingle-cell RNA sequencingTesticular tissue

Identifiers

PMID41272432
PMCPMC12751428

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.