Evidence map›Paper›PMID 41272896›Full record

ArticleVeterinary research2025

Cellular hnRNP AB inhibits avian influenza virus RNA synthesis via blocking UAP56-mediated nuclear export of PB2 mRNA.

Shuhui Liu, Yue Sun, Yunling Peng, Chenchen Xu, Suquan Song, Liping Yan

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Veterinary research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Shuhui Liu *MOE Joint International Research Laboratory of Animal Health and Food Safety, Jiangsu Engineering Laboratory of Animal Immunology, Jiangsu Detection Center of Terrestrial Wildlife Disease, Institute of Immunology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, Jiangsu Province, China.
Yue Sun *MOE Joint International Research Laboratory of Animal Health and Food Safety, Jiangsu Engineering Laboratory of Animal Immunology, Jiangsu Detection Center of Terrestrial Wildlife Disease, Institute of Immunology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, Jiangsu Province, China.
Yunling PengMOE Joint International Research Laboratory of Animal Health and Food Safety, Jiangsu Engineering Laboratory of Animal Immunology, Jiangsu Detection Center of Terrestrial Wildlife Disease, Institute of Immunology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, Jiangsu Province, China.
Chenchen XuMOE Joint International Research Laboratory of Animal Health and Food Safety, Jiangsu Engineering Laboratory of Animal Immunology, Jiangsu Detection Center of Terrestrial Wildlife Disease, Institute of Immunology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, Jiangsu Province, China.
Suquan SongMOE Joint International Research Laboratory of Animal Health and Food Safety, Jiangsu Engineering Laboratory of Animal Immunology, Jiangsu Detection Center of Terrestrial Wildlife Disease, Institute of Immunology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, Jiangsu Province, China.
Liping YanMOE Joint International Research Laboratory of Animal Health and Food Safety, Jiangsu Engineering Laboratory of Animal Immunology, Jiangsu Detection Center of Terrestrial Wildlife Disease, Institute of Immunology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, Jiangsu Province, China. yanliping@njau.edu.cn.

Funding

China Postdoctoral Science Foundation 2025M773031National Key Research and Development Program of China 2022YFC2601602National Natural Science Foundation of China 32272994Postdoctoral Fellowship Program of China Postdoctoral Science Foundation GZB20250695
6 · The paper itself

Abstract

Avian influenza viral ribonucleoproteins (vRNPs) complete genome transcription and replication by interacting with host proteins, and RNA-dependent RNA polymerase (RdRp) is its major component. PB2 is a component of RdRp and plays an important role in viral RNA synthesis. Our previous mass spectrometry analysis identified PB2 interacted with avian cellular heterogeneous nuclear ribonucleoprotein AB (hnRNP AB). However, the specific mechanism of this interaction regulating viral replication needs to be further clarified. In this study, we found that avian hnRNP AB inhibited the replication of multiple subtypes of avian influenza viruses (AIVs) from different reservoirs, and the glycine-rich domain (GRD) of hnRNP AB was the function domain that inhibited AIV replication. Moreover, we demonstrated that the GRD of avian hnRNP AB interacted with the C-terminus of PB2, reducing the binding of PB1 to PB2 and interfering with RdRp assembly. Based on the previous discovery that hnRNP AB affected the nucleoplasmic distribution of PB2 mRNA, we have further explored the mechanism here. Mechanically, hnRNP AB intervened in the nuclear export of PB2 mRNA by reducing the binding ability of UAP56, and decreased PB2 expression to interfere with RdRp formation and reduce vRNA synthesis, which in turn inhibited viral replication. Collectively, this study demonstrated that the avian host protein hnRNP AB inhibited AIV replication by blocking assembly of RdRp and vRNA synthesis, in which was associated with UAP56-mediated nuclear export of PB2 mRNA, providing a potential target for antiviral intervention.

Indexed as

Heterogeneous-Nuclear RibonucleoproteinsInfluenza A virusInfluenza in BirdsRNA-Dependent RNA PolymeraseRNA, ViralViral ProteinsVirus ReplicationAnimalsChickensRNA, MessengerHeterogeneous-Nuclear RibonucleoproteinsPB2 protein, Influenzavirus ARNA-Dependent RNA PolymeraseRNA, MessengerRNA, ViralViral ProteinsAvian influenza virushnRNP ABnuclear exportPB2vRNA synthesis

Identifiers

PMID41272896
PMCPMC12639776

What Socratic holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.