Evidence mapPaperPMID 41273001Full record

ArticleBrain and behavior2025

Vitamin D3 Improves Hypothalamic-Pituitary-Adrenal Axis Function, Immunological Responses, and Gut Dysbiosis in Sleep Desynchrony.

Changwei W Wu, Yen-Ju Huang, Jin-Wei Xu, Hsien-Yu Fan, Jun-Lan Zeng, Yu-Chen S H Yang, Yu-Tang Tung

Abstract read
In one paragraph

Article in Brain and behavior, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed, 1 pooled it
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 1 synthesis or guideline pooled it.

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4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Changwei W WuGraduate Institute of Mind, Brain and Consciousness, Taipei Medical University, Taipei, Taiwan.
Yen-Ju HuangDepartment of Pathology, Ditmanson Medical Foundation Chia-Yi Christian Hospital, Chiayi, Taiwan.
Jin-Wei XuDepartment of Forestry, National Chung Hsing University, Taichung, Taiwan.
Hsien-Yu FanSchool of Nutrition and Health Sciences, College of Nutrition, Taipei Medical University, Taipei, Taiwan.
Jun-Lan ZengGraduate Institute of Biotechnology, National Chung Hsing University, Taichung, Taiwan.
Yu-Chen S H YangJoint Biobank, Office of Human Research, Taipei Medical University, Taipei, Taiwan.
Yu-Tang TungGraduate Institute of Biotechnology, National Chung Hsing University, Taichung, Taiwan.

Funding

Ditmanson Medical Foundation Chia-Yi Christian Hospital R111-40National Health Research Institutes NHRI-EX113-11129EINational Science and Technology Council NSTC112-2314-B-038-044-MY3National Science and Technology Council NSTC112-2320-B-005-009-MY3
6 · The paper itself

Abstract

purposeChronic sleep desynchrony proliferates in modern society, characterized by extended wakefulness and disrupted circadian rhythms over a long period, and is associated with various physiological and immune dysfunctions. This study investigated the health effects of long-term sleep desynchrony and subsequent vitamin D3 treatment, alongside sleep recovery, on biological factors in C57BL/6J mice by assessing their body weight, metabolic activity, stress response, immune function, and gut microbiota composition.

methodsMice underwent 28 days of sleep desynchrony using a Lafayette chamber to model chronic disruption of the HPA axis, immune function, and gut microbiota. FINDING: Sleep desynchrony did not significantly alter body weight, food intake, or water consumption, suggesting that metabolic homeostasis was preserved. However, stress hormone analysis revealed impaired HPA axis regulation in the sleep-desynchronized mice, as indicated by suppressed corticosterone levels after dexamethasone injection. Notably, vitamin D3 treatment restored the HPA axis function by enhancing corticosterone suppression. Behavioral tests of the mice indicated that sleep desynchrony reduced nest-building ability, locomotor activity, and anxiety-like behaviors, whereas vitamin D3 treatment improved their motor function. Furthermore, vitamin D3 treatment alleviated immune dysregulation by reducing neutrophil levels and increasing lymphocyte counts, suggesting a role in restoring immune balance after sleep desynchrony. Regarding gut microbiota composition, sleep desynchrony disrupted microbial diversity, reducing Bacteroidota and increasing Firmicutes, leading to dysbiosis. Vitamin D3 treatment shifted the microbiota composition toward eubiosis, aligning it closer to control-group levels. Additionally, sleep desynchrony impaired the expression of tight junction proteins in the proximal colon, compromising intestinal barrier integrity. Vitamin D3 treatment restored the levels of key tight junction proteins, such as ZO-1 and claudin, thereby maintaining gut barrier function.

conclusionVitamin D3 treatment serves as a promising intervention for mitigating the adverse health effects of chronic sleep desynchrony on the HPA axis, immune function, and gut microbiota, providing potential therapeutic benefits for preserving physiological homeostasis in the middle of a long-term sleep deficiency or chronic sleep deprivation.

Indexed as

CholecalciferolDysbiosisGastrointestinal MicrobiomeHypothalamo-Hypophyseal SystemPituitary-Adrenal SystemAnimalsCircadian RhythmCorticosteroneMaleMiceMice, Inbred C57BLSleepSleep DeprivationCholecalciferolCorticosteronechronic sleep desynchronygut dysbiosishypothalamic–pituitary–adrenal (HPA) axisshort chain fatty acidvitamin D3

Identifiers

PMID41273001
PMCPMC12638446

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.