Evidence map›Paper›PMID 41275942›Full record

ArticleJournal of ethnopharmacology2026

Mouse neuronal dendritic complexity and resilience to stress-induced depression in Drosophila melanogaster are enhanced by Withania somnifera alkaloids.

Rudranil Dutta, Helen Holvoet, Luke Marney, Kadine Cabey, Cody Neff, Mikah Brandes, Jonathan Zweig, Jesus Martinez, Jaewoo Choi, Christine McClure and 9 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of ethnopharmacology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

19 authors.

Rudranil DuttaBENFRA Botanical Dietary Supplements Research Center, Oregon Health & Science University, Portland, OR, 97239, USA; Department of Chemistry, Oregon State University, Corvallis, OR, 97331, USA.
Helen HolvoetInstitute for Developmental Biology and Neurobiology, Johannes Gutenberg-Universität Mainz, Hanns-Dieter-Hüsch-Weg 15, 55128, Mainz, Germany.
Luke MarneyBENFRA Botanical Dietary Supplements Research Center, Oregon Health & Science University, Portland, OR, 97239, USA; Department of Chemistry, Oregon State University, Corvallis, OR, 97331, USA.
Kadine CabeyBENFRA Botanical Dietary Supplements Research Center, Oregon Health & Science University, Portland, OR, 97239, USA; Department of Neurology, Oregon Health & Science University, Portland, OR, 97239, USA.
Cody NeffBENFRA Botanical Dietary Supplements Research Center, Oregon Health & Science University, Portland, OR, 97239, USA; Department of Neurology, Oregon Health & Science University, Portland, OR, 97239, USA.
Mikah BrandesBENFRA Botanical Dietary Supplements Research Center, Oregon Health & Science University, Portland, OR, 97239, USA; Department of Neurology, Oregon Health & Science University, Portland, OR, 97239, USA.
Jonathan ZweigBENFRA Botanical Dietary Supplements Research Center, Oregon Health & Science University, Portland, OR, 97239, USA; Department of Neurology, Oregon Health & Science University, Portland, OR, 97239, USA.
Jesus MartinezBENFRA Botanical Dietary Supplements Research Center, Oregon Health & Science University, Portland, OR, 97239, USA; Department of Neurology, Oregon Health & Science University, Portland, OR, 97239, USA.
Jaewoo ChoiBENFRA Botanical Dietary Supplements Research Center, Oregon Health & Science University, Portland, OR, 97239, USA; Department of Chemistry, Oregon State University, Corvallis, OR, 97331, USA.
Christine McClureBENFRA Botanical Dietary Supplements Research Center, Oregon Health & Science University, Portland, OR, 97239, USA; Department of Neurology, Oregon Health & Science University, Portland, OR, 97239, USA.
Md Nure AlamBENFRA Botanical Dietary Supplements Research Center, Oregon Health & Science University, Portland, OR, 97239, USA; Department of Chemistry, Oregon State University, Corvallis, OR, 97331, USA.
Liping YangBENFRA Botanical Dietary Supplements Research Center, Oregon Health & Science University, Portland, OR, 97239, USA; Department of Chemistry, Oregon State University, Corvallis, OR, 97331, USA.
Burkhard PoeckInstitute for Developmental Biology and Neurobiology, Johannes Gutenberg-Universität Mainz, Hanns-Dieter-Hüsch-Weg 15, 55128, Mainz, Germany.
Jan F StevensBENFRA Botanical Dietary Supplements Research Center, Oregon Health & Science University, Portland, OR, 97239, USA; Linus Pauling Institute, Oregon State University, Corvallis, OR, 97331, USA; Department of Pharmaceutical Sciences, Oregon State University, Corvallis, OR, 97331, USA.
Doris KretzschmarBENFRA Botanical Dietary Supplements Research Center, Oregon Health & Science University, Portland, OR, 97239, USA; Oregon Institute of Occupational Health Sciences, Oregon Health & Science University, Portland, OR, 97239, USA.
Nora E GrayBENFRA Botanical Dietary Supplements Research Center, Oregon Health & Science University, Portland, OR, 97239, USA; Department of Neurology, Oregon Health & Science University, Portland, OR, 97239, USA.
Roland StraussInstitute for Developmental Biology and Neurobiology, Johannes Gutenberg-Universität Mainz, Hanns-Dieter-Hüsch-Weg 15, 55128, Mainz, Germany.
Claudia S MaierBENFRA Botanical Dietary Supplements Research Center, Oregon Health & Science University, Portland, OR, 97239, USA; Department of Chemistry, Oregon State University, Corvallis, OR, 97331, USA; Linus Pauling Institute, Oregon State University, Corvallis, OR, 97331, USA.
Amala SoumyanathBENFRA Botanical Dietary Supplements Research Center, Oregon Health & Science University, Portland, OR, 97239, USA; Department of Neurology, Oregon Health & Science University, Portland, OR, 97239, USA. Electronic address: soumyana@ohsu.edu.

Funding

Research Education ComponentP30AG066518 · NIA · OREGON HEALTH & SCIENCE UNIVERSITY · PI Lisa C Silbert · 2020 to 2026
$28.6M
CAM Research Training in Neuroscience &StressT32AT002688 · NCCIH · OREGON HEALTH & SCIENCE UNIVERSITY · PI OKEN, BARRY S, SOUMYANATH, AMALA · 2005 to 2025
$7.2M
Mechanisms underlying the cognitive effects of Centella asiatica water extract and constituent compounds in the 5xFAD mouse model of Alzheimer's DiseaseU19AT010829 · NCCIH · OREGON HEALTH & SCIENCE UNIVERSITY · PI SOUMYANATH, AMALA · 2020 to 2024
$6.1M
Ultra-Performance Liquid Chromatography Tandem Quadrupole Mass Spectrometry SystemS10OD026922 · OD · OREGON STATE UNIVERSITY · PI MAIER, CLAUDIA S · 2019 to 2019
$577k
Triple Quadrupole/Linear Ion Trap LC-MS/MS for LPIS10RR027878 · NCRR · OREGON STATE UNIVERSITY · PI STEVENS, JAN FREDERIK · 2010 to 2010
$484k
NCCIH NIH HHS T32 AT002688NCCIH NIH HHS U19 AT010829NCRR NIH HHS S10 RR027878NIA NIH HHS P30 AG066518NIH HHS S10 OD026922
6 · The paper itself

Abstract

ethnopharmacological relevanceWithania somnifera (WS), also known as ashwagandha, is used in traditional Ayurvedic medicine for its rejuvenating properties. While most research has focused on withanolides as primary bioactive constituents, this study highlights the potential role of alkaloids in the biological effects of WS. AIM OF THE STUDY: To explore compounds responsible for bioactivity of WS on dendritic complexity in mouse primary neurons and on stress-related behaviors in Drosophila melanogaster. MATERIALS AND

methodsAn aqueous extract was prepared from dried root of WS grown in Central Oregon, and fractionated into ethanol insoluble, polar, and non-polar fractions. The preparations were tested for effects on dendritic complexity of mouse primary hippocampal neurons, and on stress-induced behavioral changes in Drosophila melanogaster flies. The composition of WS extract and fractions was examined using liquid chromatography coupled to high-resolution or multiple reaction monitoring mass spectrometry (LC-HRMS/MS or LC-MRM-MS).

resultsWS root aqueous extract and its polar fraction enhanced dendritic complexity in mouse primary hippocampal neurons and significantly improved stress-related behavior in Drosophila, whereas the withanolide-rich, non-polar fraction did not. The bioactive, polar fraction contained alkaloids including acetyltropine, which was then confirmed as a major, bioactive alkaloid in WS root and differentiated from its isomer acetyl exotropine by its LC-MRM-MS retention time and 2D-NMR.

conclusionsThese findings highlight a role for alkaloids, including acetyltropine, as potential active constituents of WS associated with neuroprotective and anti-stress effects traditionally attributed to this plant. Results support continued evaluation of additional WS active compounds beyond withanolides.

Indexed as

AlkaloidsDendritesDepressionPlant ExtractsStress, PsychologicalWithaniaAnimalsBehavior, AnimalCells, CulturedDrosophila melanogasterHippocampusMaleMiceNeuronsPlant RootsAlkaloidsPlant ExtractsAcetyltropineAlkaloidsAshwagandhaDepressionDrosophilaPrimary neuronsWithanolides

Identifiers

PMID41275942
PMCPMC12875680

What Socratic holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.