Evidence map›Paper›PMID 41278395›Full record

ArticleScientifica2025

Pathology and Molecular Diagnosis of Marek's Disease Virus in Chickens in Nigeria.

Olusegun Adesina Fagbohun, Aiki-Raji Comfort Oluladun, Theophilus Aghogho Jarikre, Olugbenga Olayinka Alaka, Ademola Adetokunbo Oyagbemi, Rofiat Damilola Adesina, Oluwaseun Olanrewaju Esan, Olumide Odunayo Akinniyi, Moses Olusola Adetona, Temidayo Olutayo Omobowale and 2 more

Abstract read
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In one paragraph

Article in Scientifica, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Olusegun Adesina FagbohunDepartment of Veterinary Microbiology, Faculty of Veterinary Medicine, University of Ibadan, Ibadan, Oyo State, Nigeria.
Aiki-Raji Comfort OluladunDepartment of Veterinary Microbiology, Faculty of Veterinary Medicine, University of Ibadan, Ibadan, Oyo State, Nigeria.
Theophilus Aghogho JarikreDepartment of Veterinary Pathology, Faculty of Veterinary Medicine, University of Ibadan, Ibadan, Oyo State, Nigeria.
Olugbenga Olayinka AlakaDepartment of Veterinary Pathology, Faculty of Veterinary Medicine, University of Ibadan, Ibadan, Oyo State, Nigeria.
Ademola Adetokunbo OyagbemiDepartment of Veterinary Physiology and Biochemistry, Faculty of Veterinary Medicine, University of Ibadan, Ibadan, Oyo State, Nigeria.ORCID https://orcid.org/0000-0002-8996-8610
Rofiat Damilola AdesinaDepartment of Veterinary Microbiology, Faculty of Veterinary Medicine, University of Ibadan, Ibadan, Oyo State, Nigeria.
Oluwaseun Olanrewaju EsanDepartment of Veterinary Medicine, Faculty of Veterinary Medicine, University of Ibadan, Ibadan, Oyo State, Nigeria.
Olumide Odunayo AkinniyiDepartment of Veterinary Medicine, Faculty of Veterinary Medicine, University of Ibadan, Ibadan, Oyo State, Nigeria.ORCID https://orcid.org/0000-0002-9960-3615
Moses Olusola AdetonaDepartment of Anatomy, Faculty of Basic Medical Sciences, College of Medicine, University of Ibadan, Ibadan, Oyo State, Nigeria.
Temidayo Olutayo OmobowaleDepartment of Veterinary Medicine, Faculty of Veterinary Medicine, University of Ibadan, Ibadan, Oyo State, Nigeria.ORCID https://orcid.org/0000-0002-9486-2861
Ufuoma Joan MamohDepartment of Veterinary Microbiology, Faculty of Veterinary Medicine, University of Ibadan, Ibadan, Oyo State, Nigeria.
Ishmael Festus JajaDepartment of Livestock and Pasture Science, Faculty of Science and Agriculture, University of Fort Hare, Alice 5700, South Africa.ORCID https://orcid.org/0000-0002-9310-6511

Funding

Drug Delivery Carriers Targeted to Breast Tumor by Fusion Phage ProteinsR01CA125063 · NCI · AUBURN UNIVERSITY AT AUBURN · PI PETRENKO, VALERY A. · 2007 to 2011
$1.9M
NCI NIH HHS R01 CA125063
6 · The paper itself

Abstract

Marek's disease caused by Marek's disease virus (MDV) serotype 1 is an economically important neoplastic disease of poultry. Diagnosis of this disease is usually based on clinical signs, postmortem lesions, and diagnostic tests like cytology, histopathology, and molecular-based methods. However, there might be the problem of inaccurate diagnosis in Nigeria. Employment of gross pathology, histopathology, immunohistochemistry, and polymerase chain reaction (PCR) coupled with sequence analysis provides a reliable approach to arrive at precise confirmatory diagnosis of the disease. Therefore, visceral organs including liver, proventriculus intestine, spleen, and heart samples were collected at postmortem examinations from two pullets suspected of having Marek's disease. Histopathology and immunohistochemistry, PCR amplification of a 576 bp fragment of the MDV glycoprotein L (gL) gene, and sequence analysis were employed in this diagnostic approach. Histopathological examinations of the liver, heart and proventriculus showed neoplastic pleomorphic cellular infiltration comprising lymphoblasts, lymphocytes, macrophages, and heterophils which correspond with the pathology of Marek's disease. Liver samples were positive for the virus using PCR. Sequence analysis based on phylogenetic tree reconstruction revealed the positive MDV. MDV sequences from this study clustered with MDV serotype 1 sequences retrieved from the GenBank. This approach provides a reliable and precise diagnosis of Marek's disease in chickens which is applicable to other avian diseases.

Identifiers

PMID41278395
PMCPMC12638146

What Socratic holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.