BudFinder: A Masked Auto-Encoder Vision Transformer Framework for Yeast Budding Detection.

Yeast replicative lifespan is a crucial part of aging research, yet its quantification remains labor-intensive and time-consuming, particularly when using time-lapse imaging and microfluidics. Manual counting methods for cell division events are prone to bias and inefficiency, while existing automated approaches often require extensive annotated datasets. These limitations hinder the adaptability of such tools across different microfluidic setups. To address these challenges, we propose a versatile image analysis approach that accurately detects yeast cell division events. To reduce the burden of requiring a large cell division annotated dataset, we pretrained a Masked Auto-Encoder on large-scale segmented yeast cell images. This substantially reduced the annotated data needed to train the transformer model for detecting cellular division events. Additionally, the model is trained directly on budding event detection, circumventing reliance on arbitrary heuristics, such as changes in cell area. By leveraging self-supervised pretraining, we reduced the training data requirement to fewer than 50 mother cells (~1,000 divisions), representing a >5-fold reduction compared to prior methods while maintaining comparable accuracy.