Evidence map›Paper›PMID 41284093›Full record

ArticleSaudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society2025

Targeting triple-negative breast cancer: apoptotic and antitumor effects of Artemisia sieberi Besser extracts.

Fatimah G Albani, Sahar S Alghamdi, Sabine Matou-Nasri, Arwa Alsubait, Rasha Suliman, Rehab AlRoshody, Sarah M Huwaizi, Tlili Barhoumi, Afrah E Mohammed, Mohamed Boudjelal and 2 more

Abstract read
In one paragraph

Article in Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Design, optimization and characterization of mucoadhesive buccal film as a novel delivery system for doxazosin mesylate.Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Fatimah G AlbaniDepartment of Zoology, College of Sciences, King Saud University, Riyadh, Saudi Arabia.
Sahar S AlghamdiKing Abdullah International Medical Research Center, Riyadh, Saudi Arabia.
Sabine Matou-NasriKing Abdullah International Medical Research Center, Riyadh, Saudi Arabia.
Arwa AlsubaitKing Abdullah International Medical Research Center, Riyadh, Saudi Arabia.
Rasha SulimanFatima College for Health Sciences, Abu Dhabi, United Arab Emirates.
Rehab AlRoshodyKing Abdullah International Medical Research Center, Riyadh, Saudi Arabia.
Sarah M HuwaiziKing Abdullah International Medical Research Center, Riyadh, Saudi Arabia.
Tlili BarhoumiKing Abdullah International Medical Research Center, Riyadh, Saudi Arabia.
Afrah E MohammedDepartment of Biology, College of Sciences, Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia.
Mohamed BoudjelalKing Abdullah International Medical Research Center, Riyadh, Saudi Arabia.
Entissar S AlsuhaibaniDepartment of Zoology, College of Sciences, King Saud University, Riyadh, Saudi Arabia.
Zeyad AlehaidebKing Abdullah International Medical Research Center, Riyadh, Saudi Arabia. alehaidebze1@MNGHA.MED.SA.ORCID http://orcid.org/0000-0002-7185-2820

Funding

King Abdullah International Medical Research Center RC16/175/R
6 · The paper itself

Abstract

Artemisia sieberi Besser (A. sieberi) has shown promise as a natural source of safe cytotoxic agents for breast cancer therapy. However, its effects on triple-negative breast cancer (TNBC), a very aggressive subtype lacking targeted treatments, remain poorly studied. This study evaluates the anticancer activity of A. sieberi extracts against the MDA-MB-231 TNBC cell line, comparing results with hormone receptor-positive MCF-7 cells. Aerial parts of A. sieberi were extracted using ethanol and methanol and then chemically characterized via gas chromatography/mass spectrometry (GC/MS) to identify bioactive compounds. Cytotoxic effects were assessed using cell viability assays, while apoptosis induction was examined through flow cytometry (Annexin V/propidium iodide staining), caspase-3/-7 activation assays, and mitochondrial membrane permeabilization visualized using confocal microscopy. Western blotting analyzed the expression levels of apoptotic proteins. In silico molecular docking simulations explored the interactions between phytochemicals and apoptosis-regulating proteins. A. sieberi extracts exhibited higher cytotoxicity against MDA-MB-231 cells than against MCF-7 cells, with lower IC

Indexed as

ApoptosisCytotoxicityMCF-7MDA-MB-231Molecular dockingPhytochemicalsTriple-negative breast cancer

Identifiers

PMID41284093
PMCPMC12644357

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.