ArticleBMC plant biology2025
Characterization and genetic dissection of the powdery mildew resistance in cultivated emmer wheat accession L424.
Article in BMC plant biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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1 citing paper in PubMed.
- Identification and characterization of the powdery mildew resistance in a spelt accession Lsy-93.BMC plant biology · 2025Article
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Abstract
backgroundPowdery mildew, caused by the fungal pathogen Blumeria graminis f. sp. tritici (Bgt), is a widespread disease affecting wheat globally, exerting significant adverse impacts on both wheat yield and quality. The utilization of powdery mildew resistance genes represents the most effective and sustainable strategy for managing this disease. Cultivated emmer wheat (T. dicoccum, 2n = 4x = 28, AABB), recognized as one of the key ancestral species of common wheat, has emerged as a valuable genetic resource for enhancing powdery mildew resistance in modern wheat breeding programs.
resultsThe cultivated emmer wheat accession L424 exhibits a high level of resistance to multiple Bgt isolates under both seedling and adult stages. In this study, a novel gene PmL424 underlying this resistance was identified in L424. Using bulked segregant RNA sequencing (BSR-Seq) and molecular marker analysis, PmL424 was mapped to a 3.9 cM genetic interval on chromosome arm 2BS, flanked by the markers YTU424-L99 and YTU424-L10, corresponding to a physical interval of 1.09 Mb (21.63-22.72 Mb). PmL424 likely represents a new allele of Pm68, given its origin, resistance spectrum, and physical map with previously reported resistance genes on a similar chromosomal interval. Seven genes in this interval were associated with disease resistance and were considered as the candidate genes of PmL424. BSR-Seq analysis further revealed 2,325 differentially expressed genes (DEGs) between resistant and susceptible bulks, which were then used for further Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis. qPCR analysis of seven disease resistance-associated genes within the candidate interval demonstrated that three genes TRITD2Bv1G010130, TRITD2Bv1G010140 and TRITD2Bv1G010240 were significantly up-regulated in L424 compared to the susceptible parent Langdon after inoculating the Bgt isolate E09. Further analysis revealed that only genes TRITD2Bv1G010130 and TRITD2Bv1G010240 exhibit sequence variations between resistant and susceptible genotypes. Validation using 46 susceptible wheat accessions confirmed that the marker YTU024-L99 could accurately trace the presence of PmL424, providing practical tools for marker-assisted selection breeding of PmL424.
conclusionsIn the present study, a Pm gene PmL424 was identified using genetic analysis, BSR-Seq analysis and molecular markers in the cultivated emmer wheat accession L424, and three key candidate genes were also captured through time-course analysis post inoculation with Bgt isolate E09. Potential regulatory genes were also identified through DEG analysis using GO and KEGG pathway analysis, unraveling their potential transcriptional regulation mechanisms when fighting Bgt invasion. These multi-dimensional analyses provide valuable insights into the dissection of the molecular and genetic basis underlying the powdery mildew resistance, as well as establishing a foundation for MAS using PmL424.
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