ArticleMicrobial biotechnology2025
Biocontrol of Root-Knot Nematodes via siRNA-Loaded Extracellular Vesicles From a Nematophagous Fungus Arthrobotrys oligospora.
Article in Microbial biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
3 citing papers in PubMed.
- Bacterial Extracellular Vesicles in Biomedical Research and Clinical Translation.Microbial biotechnology · 2026Review
- Functional diversity and evolutionary strategies in nematophagous fungi and plant parasitic nematodes interactions - a review.Frontiers in plant science · 2026Review
- Biocontrol of Root-Knot Nematodes via siRNA-Loaded Extracellular Vesicles From a Nematophagous Fungus Arthrobotrys oligospora.Microbial biotechnology · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
Root-knot nematodes (Meloidogyne spp.) represent a major threat to global crop production, and current chemical nematicides pose serious environmental and health risks. RNA interference (RNAi) offers a promising gene-specific strategy for nematode control. However, the efficient and sustainable delivery of RNA molecules into nematodes remains a significant challenge. In this study, we developed an innovative RNA delivery platform using extracellular vesicles (EVs) derived from the nematode-trapping fungus Arthrobotrys oligospora. EVs were either exogenously loaded with synthetic siRNAs targeting the Mi-flp-18 gene of M. incognita or harvested from engineered fungal strains expressing short hairpin RNAs (shRNAs) or double-stranded RNAs (dsRNAs) against multiple nematode neuropeptide genes (flp and nlp families). The engineered EVs efficiently delivered RNA cargos into nematodes, leading to significant downregulation of target gene expression. Functional assays and greenhouse experiments revealed the biocontrol potential of the engineered fungal strains, with reductions in nematode motility, root invasion and infectivity. This is the first demonstration in a nematophagous fungus that EVs can serve as effective RNA delivery vehicles for the control of root-knot nematodes. The use of engineered A. oligospora strains provides a scalable, eco-friendly alternative to synthetic delivery systems and transgenic crops. Our findings establish fungal EVs as a powerful tool in cross-kingdom RNAi applications and open new avenues for sustainable pest management in agriculture.
Indexed as
Identifiers
What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.