Evidence map›Paper›PMID 41288403›Full record

ArticlemSphere2025

Imaging flow cytometry enables label-free cell sorting of morphological variants from populations of the unculturable bacterium

Daniel Vocelle, Lindsey Thompson, Matthew P Bernard, Nina Wale

Abstract read
In one paragraph

Article in mSphere, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Daniel VocelleDepartment of Pharmacology and Toxicology, Michigan State University, East Lansing, Michigan, USA.
Lindsey ThompsonDepartment of Microbiology, Genetics and Immunology, Michigan State University, East Lansing, Michigan, USA.
Matthew P BernardDepartment of Pharmacology and Toxicology, Michigan State University, East Lansing, Michigan, USA.
Nina WaleDepartment of Pharmacology and Toxicology, Michigan State University, East Lansing, Michigan, USA.ORCID 0000-0001-9703-444X

Funding

College of Natural Science, Michigan State UniversityU.S. Department of Agriculture 2022-70410-38419
6 · The paper itself

Abstract

Bacterial populations often display remarkable morphological heterogeneity. Fluorescence-activated cell sorting (FACS) is an important tool for understanding this diversity. FACs allows researchers to obtain pure samples of each morphological variant (or morphotype) that is present within a mixed population of cells and thus permits each morphotype to be phenotyped. In FACS, cells are first labeled with fluorescent markers, such as antibodies or transgenic constructs, and then separated out based on their possession of these labels. However, since the development of fluorescent labels requires

Indexed as

Flow CytometryImage Cytometryautofluorescencecell sortingimaging flow cytometrynon-model systemPasteuria ramosa

Identifiers

PMID41288403
PMCPMC12724247

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.