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ArticleNaunyn-Schmiedeberg's archives of pharmacology2026

Evaluation of 1H-pyrazol-1-yl benzenesulfonamide derivatives in alleviating inflammatory bowel disease via PI3K/Akt/mTOR signaling pathway in zebrafish models.

S P Ramya Ranjan Nayak, D Priya, V Chitra, Ajay Guru, Mohamed Farouk Elsadek, Mohamed S Elshikh, Selvaraj Arokiyaraj, M K Kathiravan, Jesu Arockiaraj

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Article in Naunyn-Schmiedeberg's archives of pharmacology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

S P Ramya Ranjan NayakToxicology and Pharmacology Laboratory, Department of Biotechnology, Faculty of Science and Humanities, SRM Institute of Science and Technology, Kattankulathur, 603203, Chengalpattu District, Tamil Nadu, India.
D PriyaDr APJ Abdul Kalam Research Lab, Department of Pharmaceutical Chemistry, SRM College of Pharmacy, Faculty of Medicine and Health Sciences, SRM Institute of Science and Technology, Kattankulathur, 603203, Chengalpattu District, Tamil Nadu, India.
V ChitraDepartment of Pharmacology, SRM College of Pharmacy, Faculty of Medicine and Health Sciences, SRM Institute of Science and Technology, Kattankulathur, 603203, Chengalpattu District, Tamil Nadu, India.
Ajay GuruDepartment of Pharmacy, School of Medical and Allied Sciences, K.R. Mangalam University, Gurugram, India. ajayguru040996@gmail.com.
Mohamed Farouk ElsadekDepartment of Biochemistry, College of Science, King Saud University, P.O. 2455, 11451, Riyadh, Saudi Arabia.
Mohamed S ElshikhDepartment of Botany and Microbiology, College of Science, King Saud University, P.O. 2455, 11451, Riyadh, Saudi Arabia.
Selvaraj ArokiyarajDepartment of Food Science and Biotechnology, Sejong University, Seoul, 05006, South Korea.
M K KathiravanDr APJ Abdul Kalam Research Lab, Department of Pharmaceutical Chemistry, SRM College of Pharmacy, Faculty of Medicine and Health Sciences, SRM Institute of Science and Technology, Kattankulathur, 603203, Chengalpattu District, Tamil Nadu, India. kathirak@srmist.edu.in.
Jesu ArockiarajToxicology and Pharmacology Laboratory, Department of Biotechnology, Faculty of Science and Humanities, SRM Institute of Science and Technology, Kattankulathur, 603203, Chengalpattu District, Tamil Nadu, India. jesuaroa@srmist.edu.in.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The incidence of inflammatory bowel disease (IBD) is increasing in developed nations, leading to a significant increase in healthcare expenses. It is characterized by chronic intestinal inflammation driven by excessive oxidative stress and dysregulated immune responses, leading to epithelial injury and impaired mucosal healing. Existing treatments have adverse effects, patient noncompliance issues, and high costs, emphasizing the need for new treatments. Pyrazole derivatives are promising antioxidant and anti-inflammatory bioactive compounds. This study uses zebrafish to test 1H-pyrazol-1-yl benzenesulfonamide derivatives for IBD treatment. Through network pharmacology and molecular docking, various 1H-pyrazol-1-yl benzenesulfonamide derivatives were screened, and T8 (4-(5-(4-chlorophenyl)-3-(2-(4-phenoxybenzylidene)hydrazine-1-carbonyl)-1H-pyrazol-1-yl)benzenesulfonamide) was chosen for in vitro and in vivo evaluation. T8 at 200 μM exhibited dose-dependent antioxidant (> 80%) and anti-inflammatory (> 85%) activities in vitro. In the zebrafish intestine, administering T8 at 150 μM led to a reduction in reactive oxygen species (ROS) (2.97-fold), lipid peroxidation, cellular apoptosis (2.30-fold), and macrophage accumulation. It restored superoxide dismutase (SOD) (19.49 U/mg) and catalase (CAT) (0.54 U/mg) levels while reducing malondialdehyde (MDA) (0.44 μmol), lactate dehydrogenase (LDH) (0.23 U/mg), and nitric oxide (NO) (3.61 μmol) levels. The expression levels of proinflammatory cytokines in zebrafish intestines were significantly reduced due to T8 suppressing the PI3K/Akt/mTOR signaling pathway. The gut histoarchitecture was restored after T8 treatment, as shown by histological analysis. By modulating the PI3K/Akt/mTOR signaling pathway, T8 reduced inflammation and oxidative stress in zebrafish, reducing IBD symptoms.

Indexed as

Anti-Inflammatory AgentsAntioxidantsInflammatory Bowel DiseasesPyrazolesSulfonamidesAnimalsBenzenesulfonamidesDisease Models, AnimalMolecular Docking SimulationOxidative StressPhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktSignal TransductionTOR Serine-Threonine KinasesZebrafishAnti-Inflammatory AgentsAntioxidantsBenzenesulfonamidesPhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktPyrazolesSulfonamidesTOR Serine-Threonine KinasesAnti-inflamationInflammatory bowel diseaseNetwork pharmacologyPI3K/Akt/mTOR signalingPyrazole derivative

Identifiers

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.