Evidence map›Paper›PMID 41288802›Full record

ArticleVeterinary research communications2025

Lactococcus lactis MSH-08 and Leuconostoc citreum MSH-09: A probiotic enhancing growth, immunity, and intestinal gene expression in broiler chickens.

Maha A Aljumaa, Salah E Maaty, Mahmoud Madkour, Mohamed M Elashtokhy, Mamdouh K Amin, Ahmed S Hassan, Dalal Sulaiman Alshaya, Fatmah A Safhi, Eman Fayad, Fatimah Hadadi and 2 more

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Article in Veterinary research communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Maha A AljumaaDepartment of Biology, College of Science, Princess Nourah bint Abdulrahman University, Riyadh, 11671, Saudi Arabia.
Salah E MaatyDepartment of Genetics, Faculty of Agriculture, Zagazig University, Zagazig, 44511, Egypt. semaaty@zu.edu.eg.
Mahmoud MadkourAnimal production Department, National Research Centre, Giza, Egypt.
Mohamed M ElashtokhyDepartment of Genetics, Faculty of Agriculture, Zagazig University, Zagazig, 44511, Egypt.
Mamdouh K AminDepartment of Genetics, Faculty of Agriculture, Zagazig University, Zagazig, 44511, Egypt.
Ahmed S HassanDepartment of Genetics, Faculty of Agriculture, Zagazig University, Zagazig, 44511, Egypt.
Dalal Sulaiman AlshayaDepartment of Biology, College of Science, Princess Nourah bint Abdulrahman University, Riyadh, 11671, Saudi Arabia.
Fatmah A SafhiDepartment of Biology, College of Science, Princess Nourah bint Abdulrahman University, Riyadh, 11671, Saudi Arabia.
Eman FayadDepartment of Biotechnology, College of Sciences, Taif University, Taif, 21944, Saudi Arabia.
Fatimah HadadiDepartment of biology, Faculty of Science, Al-Baha University, Al-Baha,, 65527, Kingdom of Saudi Arabia.
Ahmad F AlhomodiDepartment of Biology College of Science and Arts, Najran University , Najran, Kingdom of Saudi Arabia.
Samir MahgoubAgricultural Microbiology Department, Faculty of Agriculture, Zagazig University, Zagazig, 44511, Egypt. mahgoubsamir@gmail.com.

Funding

Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia PNURSP2025R465
6 · The paper itself

Abstract

This study evaluated the potential probiotic cocci of Lactococcus lactis MSH-08 and Leuconostoc citreum MSH-09, both individually and in combination, as alternatives to antibiotics for enhancing growth performance, immune response, blood lipid profiles, intestinal epithelial integrity, and gene expression in broiler chickens. L. lactis produced the highest concentration of lactic acid, while L. citreum had the highest production of acetic acid, butyric acid, and propionic acid. The strains were incorporated into feed at a level of 1.4 × 109 CFU/mL (2.0 mL/kg of diet). A total of 320 Cobb-500 broiler chicks (7 days old) were randomly assigned to four dietary groups: a control group (basal diet), a group receiving L. lactis MSH-08, a group receiving L. citreum MSH-09, and a group receiving the combination of both probiotics. The results showed that L. lactis MSH-08 significantly improved body weight at 3 and 6 weeks and reduced feed conversion ratio during the first three weeks. The combination of both probiotics did not provide additional benefits. L. lactis MSH-09 also significantly lowered aspartate transaminase (AST) and creatinine levels, increased antioxidant capacity and immune response, and reduced uric acid levels compared to the control group. Furthermore, it improved intestinal morphology by increasing villus height and the villus height-to-crypt depth ratio in the duodenum at 6 weeks. Gene expression analysis revealed that the tumor necrosis factor alpha (TNF), Interleukin-8 (IL-8), Mucin 2 (MUC2), claudin-1 (CLDN1), and tight junction protein 1 (TJP1) were highly upregulated in the jejunum of L. lactis MSH-08 group. These findings suggest that L. lactis MSH-08 may be useful probiotic for improving growth performance and intestinal health of broilers without the use of antibiotics in poultry farming.

Indexed as

ChickensGene ExpressionLactococcus lactisLeuconostocProbioticsAnimal FeedAnimalsDietGene Expression RegulationIntestinesMaleRandom AllocationGene expressionGrowth ratesGut healthLactococcus lactisLeuconostoc citreumProbiotics

Identifiers

PMID41288802

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.