Evidence map›Paper›PMID 41296180›Full record

ArticleMolecular biology reports2025

Molecular dissection of actin depolymerizing factors in wheat and its progenitors: their dynamic expression under elevated temperature stress.

Dipti Kumari, Ishita Banerjee, Alok Jain, Kunal Mukhopadhyay

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Article in Molecular biology reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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5 · Who and what money

Authors and funding

4 authors.

Dipti Kumari *Department of Bioengineering and Biotechnology, Birla Institute of Technology, Mesra, Ranchi, 835215, Jharkhand, India.
Ishita Banerjee *Department of Bioengineering and Biotechnology, Birla Institute of Technology, Mesra, Ranchi, 835215, Jharkhand, India.
Alok JainDepartment of Bioengineering and Biotechnology, Birla Institute of Technology, Mesra, Ranchi, 835215, Jharkhand, India.
Kunal MukhopadhyayDepartment of Bioengineering and Biotechnology, Birla Institute of Technology, Mesra, Ranchi, 835215, Jharkhand, India. kmukhopadhyay@bitmesra.ac.in.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundWheat (Triticum aestivum L.) is a crucial cereal crop cultivated worldwide. However, sustainable wheat production is confronted with several environmental threats, including climate change-induced temperature rise, which alters morpho-physiological traits, thereby adversely affects yield. To survive under elevated temperatures, plants have developed defense mechanisms like maintaining cytoskeleton dynamics modulated by Actin Depolymerizing Factors (ADFs). However, the molecular response of ADFs to elevated temperatures in wheat and its progenitors [Triticum durum and Aegilops tauschii] are not yet elucidated. METHODS AND

resultsThirty five potential ADF genes were identified and studied in wheat and its progenitors for phylogenetic relationships, gene structures, motif analysis, chromosomal distribution, gene ontology and cis-elements. Light, phytohormone and abiotic stress responsive cis-elements were copious. Two segmental duplicated and six tandemly duplicated ADF gene pairs were determined. Furthermore, these genes displayed a rich syntenic relationship across Oryza sativa and Brachypodium distachyon. The protein-protein interaction network revealed two hub-proteins: WD-repeat domain containing and Adenylyl cyclase-associated proteins which play essential roles in the reorganization of cytoskeleton dynamics and actin depolymerization respectively. Expression analysis demonstrated differential gene expression patterns among wheat and its progenitors, with Triticum durum displaying higher expression levels and quick recovery in response to elevated temperatures. Several Aegilops and wheat microRNAs targeted ADF genes and displayed inverse regulation under elevated temperatures at the post-transcriptional level.

conclusionsOur results provide a comprehensive understanding of ADF genes in wheat and its progenitors in response to elevated temperature suggesting that genetic manipulation could be a promising avenue for enhancing resilience.

Indexed as

Actin Depolymerizing FactorsTriticumActinsAegilopsGene Expression ProfilingGene Expression Regulation, PlantOryzaPhylogenyPlant ProteinsStress, PhysiologicalTemperatureActin Depolymerizing FactorsActinsPlant ProteinsActin depolymerising factorAegilops tauschiiElevated temperature stressTriticum aestivumTriticum durum

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.