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ArticleProbiotics and antimicrobial proteins2026

Exploring the Probiotic Potential and Immunomodulatory Capacity of Lactococcus lactis NCDO 2118 Against Bacterial Pathogens and Trypanosoma cruzi.

Mariana Oliveira Silva, Malu Mateus Santos Obata, Rafael Obata Trevisan, Chamberttan Souza Desidério, Hugo Felix Perini, Weslley Guimarães Bovi, Isabella de Oliveira Ferrato Sousa, Isabela Sguilla Rotta, Marcela Fernandes da Matta, Julia de Moura Bernardi and 13 more

Abstract read
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In one paragraph

Article in Probiotics and antimicrobial proteins, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

23 authors.

Mariana Oliveira Silva *Department of Immunology, Microbiology and Parasitology, Federal University of Triângulo Mineiro, Uberaba, MG, Brazil.
Malu Mateus Santos Obata *Department of Immunology, Microbiology and Parasitology, Federal University of Triângulo Mineiro, Uberaba, MG, Brazil. malumateus26@gmail.com.
Rafael Obata TrevisanDepartment of Immunology, Microbiology and Parasitology, Federal University of Triângulo Mineiro, Uberaba, MG, Brazil.
Chamberttan Souza DesidérioDepartment of Immunology, Microbiology and Parasitology, Federal University of Triângulo Mineiro, Uberaba, MG, Brazil.
Hugo Felix PeriniDepartment of Immunology, Microbiology and Parasitology, Federal University of Triângulo Mineiro, Uberaba, MG, Brazil.
Weslley Guimarães BoviDepartment of Immunology, Microbiology and Parasitology, Federal University of Triângulo Mineiro, Uberaba, MG, Brazil.
Isabella de Oliveira Ferrato SousaDepartment of Immunology, Microbiology and Parasitology, Federal University of Triângulo Mineiro, Uberaba, MG, Brazil.
Isabela Sguilla RottaDepartment of Immunology, Microbiology and Parasitology, Federal University of Triângulo Mineiro, Uberaba, MG, Brazil.
Marcela Fernandes da MattaDepartment of Immunology, Microbiology and Parasitology, Federal University of Triângulo Mineiro, Uberaba, MG, Brazil.
Julia de Moura BernardiDepartment of Immunology, Microbiology and Parasitology, Federal University of Triângulo Mineiro, Uberaba, MG, Brazil.
Nadine Aparecida de Souza BeneditoDepartment of Immunology, Microbiology and Parasitology, Federal University of Triângulo Mineiro, Uberaba, MG, Brazil.
Anna Victória Bernandes E BorgesDepartment of Immunology, Microbiology and Parasitology, Federal University of Triângulo Mineiro, Uberaba, MG, Brazil.
Letícia de Castro OliveiraLaboratory of Cellular and Molecular Genetics, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.
Alessandra Barbosa Ferreira MachadoDepartment of Parasitology Microbiology, Immunology and Institute of Biological Science, Federal University of Juiz de Fora, Juiz de Fora, MG, Brazil.
Aline Dias PaivaDepartment of Immunology, Microbiology and Parasitology, Federal University of Triângulo Mineiro, Uberaba, MG, Brazil.
Virmondes Rodrigues JuniorDepartment of Immunology, Microbiology and Parasitology, Federal University of Triângulo Mineiro, Uberaba, MG, Brazil.
Carlo José Freire de OliveiraDepartment of Immunology, Microbiology and Parasitology, Federal University of Triângulo Mineiro, Uberaba, MG, Brazil.
Helioswilton Sales-CamposInstitute of Tropical Pathology and Public Health, Federal University of Goiás, Goiânia, GO, Brazil.
Lúcio Roberto Cançado CastellanoCell Culture and Analysis Laboratory, Federal University of Paraíba, Paraíba, PB, Brazil.
Siomar de Castro SoaresDepartment of Immunology, Microbiology and Parasitology, Federal University of Triângulo Mineiro, Uberaba, MG, Brazil.
Juliana Reis MachadoDepartment of Immunology, Microbiology and Parasitology, Federal University of Triângulo Mineiro, Uberaba, MG, Brazil.
Vasco Ariston de Carvalho AzevedoLaboratory of Cellular and Molecular Genetics, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.
Marcos Vinicius da SilvaDepartment of Immunology, Microbiology and Parasitology, Federal University of Triângulo Mineiro, Uberaba, MG, Brazil.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Probiotic microorganisms capable of modulating the immune response and exerting antimicrobial activity represent promising tools in host-pathogen control. This study aimed to evaluate the probiotic potential and immunomodulatory capacity of Lactococcus lactis NCDO 2118 (L. lactis NCDO 2118) and the cell-free supernatant (CFS) against pathogenic bacteria and Trypanosoma cruzi (T. cruzi). We assessed acid and bile salt resistance, adhesion to abiotic surfaces, inhibition of bacterial growth, cytotoxicity, and immunomodulatory activity in vitro using RAW 264.7, THP-1, and BMDC cell lines. In vivo, C57BL/6 mice were treated orally to investigate systemic immunomodulatory responses. The strain exhibited tolerance to pH 3.5 and 0.3% bile salts, also showed adhesion capacity on polystyrene surface, and inhibited the growth of Escherichia coli, Staphylococcus aureus, Enterococcus faecalis, Enterococcus faecium, and Enterococcus gallinarum. Neither the bacterium nor its CFS exhibited cytotoxic effects. In vitro, assays showed modulation of cytokine production, especially increased IL-10 in BMDCs (bone marrow-derived dendritic cells) and reduced IL-4 in T. cruzi-infected macrophages, while in vivo assays revealed increased IL-10 levels in spleens and TNF-α in livers of treated mice. These findings highlight L. lactis NCDO 2118 as a promising probiotic candidate with dual antimicrobial and immunomodulatory actions. To our knowledge, this is the first study to demonstrate the ability of L. lactis NCDO 2118 to modulate cytokine profiles and antiparasitic responses relevant to Chagas disease.

Indexed as

Chagas DiseaseImmunologic FactorsLactococcus lactisProbioticsTrypanosoma cruziAnimalsCytokinesEscherichia coliHumansMiceMice, Inbred C57BLStaphylococcus aureusCytokinesImmunologic FactorsCFSCytokinesImmunomodulationLactic acid bacteriaProbioticTrypanosoma cruzi

Identifiers

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.