Evidence map›Paper›PMID 41299221›Full record

ArticleBMC microbiology2025

The very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase Phs1 regulates ATP levels and virulence in Cryptococcus neoformans.

Yuanyuan Ma, Kaisu Pan, Bin Xu, Wenjie Fang, Weihua Pan, Wanqing Liao, Cunwei Cao, Peng Xue, Liuyang Cai

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Yuanyuan Ma *Nantong Key Laboratory of Environmental Toxicology, Department of Occupational Medicine and Environmental Toxicology, School of Public Health, Nantong University, Nantong, 226019, China. myycsd@ntu.edu.cn.
Kaisu Pan *Department of Dermatology and Venereology, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, China.
Bin Xu *4Jiangxi Key Laboratory of oncology(2024SSY06041), JXHC Key Laboratory of Tumor Metastasis, Jiangxi Cancer Hospital, The Second Affiliated Hospital of Nanchang Medical College, Nanchang, 330029, China.
Wenjie FangDepartment of Dermatology, Shanghai Key Laboratory of Molecular Medical Mycology, Shanghai Changzheng Hospital, Naval Medical University, Shanghai, 200003, China.
Weihua PanDepartment of Dermatology, Shanghai Key Laboratory of Molecular Medical Mycology, Shanghai Changzheng Hospital, Naval Medical University, Shanghai, 200003, China.
Wanqing LiaoDepartment of Dermatology, Shanghai Key Laboratory of Molecular Medical Mycology, Shanghai Changzheng Hospital, Naval Medical University, Shanghai, 200003, China.
Cunwei CaoDepartment of Dermatology and Venereology, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, China.
Peng XueNantong Key Laboratory of Environmental Toxicology, Department of Occupational Medicine and Environmental Toxicology, School of Public Health, Nantong University, Nantong, 226019, China. pengxue@ntu.edu.cn.
Liuyang CaiDepartment of Dermatology, Shanghai Key Laboratory of Molecular Medical Mycology, Shanghai Changzheng Hospital, Naval Medical University, Shanghai, 200003, China. liuyang_cai@126.com.

Funding

Guangxi Science and Technology Major Program Guike AB24010134Nantong Jiangsu Scientific Research Project JC2023043Natural Science Foundation of Jiangsu Province BK20240948Natural Science Research of Jiangsu Higher Education Institutions 24KJD430010
6 · The paper itself

Abstract

Cryptococcus neoformans is a significant fungal pathogen that poses a serious threat to immunocompromised individuals, particularly those with HIV/AIDS. In the study, we found that the very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase, Phs1, was important for melanin formation, growth at 39 °C, maintenance of cell wall integrity, and virulence in murine models. Through RNA sequencing, we examined transcriptomic alterations and discovered that the deletion of PHS1 resulted in the upregulation of 169 genes and downregulation of 540 genes, many of which are associated with critical metabolic and cellular functions. Notably, the PHS1-deficient mutant exhibited a significant reduction in ATP-dependent activities compared to wild-type cells at mRNA level. Furthermore, metabolomic data indicated considerable disruptions in oxidative phosphorylation and the TCA cycle following PHS1 deletion. Collectively, our findings underscore the potential role of Phs1 in cellular energy regulation and its contribution to the virulence of C. neoformans.

Indexed as

Adenosine TriphosphateCryptococcus neoformansEnoyl-CoA HydrataseFungal ProteinsHydro-LyasesAnimalsCell WallCryptococcosisDisease Models, AnimalFemaleGene DeletionGene Expression Regulation, FungalMelaninsMiceVirulenceAdenosine TriphosphateEnoyl-CoA HydrataseFungal ProteinsHydro-LyasesMelaninsATPATP-dependent activitiesCryptococcosisPhs1Virulence

Identifiers

PMID41299221
PMCPMC12659266

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.