Evidence mapPaperPMID 41300467Full record

ArticleAntioxidants (Basel, Switzerland)2025

Oxidative Stress and NLRP3 Inflammasome as Markers of Cardiac Injury Following Cardiopulmonary Bypass: Potential Implications for Patients with Preoperative Heart Failure with Reduced Ejection Fraction.

Rodrigo L Castillo, Rodrigo A Carrasco, Alejandro Gonzaléz-Candia, Esteban G Figueroa, Adolfo A Paz, Alejandro A Candia, Sawa Kostin, Nikolaos Pagonas, Pamela V Arias, Emilio A Herrera and 2 more

Abstract read
In one paragraph

Article in Antioxidants (Basel, Switzerland), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Rodrigo L CastilloDepartamento de Medicina Interna Oriente, Facultad de Medicina, Universidad de Chile, Santiago 7500922, Chile.ORCID 0000-0002-0549-1326
Rodrigo A CarrascoDepartment of Cardiology, University Heart Center, University Hospital Zurich, Univesity of Zurich, 8091 Zurich, Switzerland.ORCID 0000-0001-9543-8619
Alejandro Gonzaléz-CandiaInstitute of Health Sciences, University of O'Higgins, Rancagua 3655000, Chile.
Esteban G FigueroaEscuela de Obstetricia, Facultad de Ciencias para el Cuidado de la Salud, Universidad San Sebastián, Santiago 7510157, Chile.ORCID 0000-0003-1316-738X
Adolfo A PazLaboratory of Vascular Function and Reactivity, Pathophysiology Program, Institute of Biomedical Sciences (ICBM), Faculty of Medicine, Universidad de Chile, Santiago 8330015, Chile.ORCID 0000-0003-4611-2748
Alejandro A CandiaDepartment for the Woman and Newborn Health Promotion, Universidad de Chile, Santiago 8330015, Chile.
Sawa KostinFaculty of Health Sciences, Brandenburg Medical School Theodor Fontane, 16816 Neuruppin, Germany.
Nikolaos PagonasDepartment of Cardiology, Medical School Theodor Fontane, University Hospital Ruppin-Brandenburg, 16816 Neuruppin, Germany.ORCID 0000-0003-3226-8135
Pamela V AriasLaboratory of Vascular Function and Reactivity, Pathophysiology Program, Institute of Biomedical Sciences (ICBM), Faculty of Medicine, Universidad de Chile, Santiago 8330015, Chile.
Emilio A HerreraLaboratory of Vascular Function and Reactivity, Pathophysiology Program, Institute of Biomedical Sciences (ICBM), Faculty of Medicine, Universidad de Chile, Santiago 8330015, Chile.
Robert A PérezSchool of Medicine, Universidad de los Andes, Santiago 7620001, Chile.ORCID 0009-0009-8835-7525
Sebastián IturraInstituto Nacional del Tórax, Santiago 7510150, Chile.

Funding

FONDECYT grant 1241502
6 · The paper itself

Abstract

Cardiopulmonary bypass (CPB) can lead to cardiac damage due to oxidative stress (OS) and inflammation in heart failure (HF). We tested the hypothesis that preoperative HF patients with reduced ejection fraction (HFrEF) subjected to CBP have higher levels of OS and NLRP3 (NOD-, LRR- and pyrin domain-containing protein 3) in heart and plasma and in those that develop postoperative AF (pAF) as a clinical outcome. HF was categorized for preoperative left ventricular EF: preserved (HFpEF > 50%, n = 27) and reduced EF (HFrEF ≤ 40%, n = 25). Samples of atrial tissue, pericardial fluid, and plasma were collected at surgery to assess NLRP3 expression; 3-nitrotyrosine (3-NT), thiobarbituric acid reaction (TBARS), and nuclear factor erythroid 2-related factor 2 (Nrf2) in atrial tissue; NLRP3, IL-1β, and IL-18 expression in pericardial fluid; and antioxidant capacity, 8-isoprostanes, and malondialdehyde (MDA) in plasma. Reactive oxygen species, 3-NT, and NLRP3 in atrial tissue were determined by immunohistochemistry in a subset of pAF patients. Plasma and atrial tissue 3-NT and MDA were higher in HFrEF compared with HFpEF. Lipid peroxidation products were higher in both plasma and atrial tissue in pAF (n = 29), compared to sinus rhythm (SR) (n = 23). In HFrEF patients, the values of tissue ROS, 3-NT, and NLRP3 were higher than in HFpEF patients. In addition, the expression levels of NLRP3, IL-1β, and IL-18 were higher in atrial tissue and pericardial fluid in HFrEF. Patients with preoperative HFrEF showed higher OS in plasma and the expression of NLRP3, ROS, and 3-NT in atrial tissue biopsies and pericardial fluid. This finding suggests a potential pharmacologic therapy for pAF and clinical complications due to CPB.

Indexed as

3-nitrotyrosineheart failure with reduced left ventricular ejection fractionHF with preserved ejection fractionNLRP3oxidative stress markerspostoperative atrial fibrillation

Identifiers

PMID41300467
PMCPMC12649429

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.