Evidence map›Paper›PMID 41301171›Full record

ArticleBioengineering (Basel, Switzerland)2025

Enhanced U-Net-Based Deep Learning Model for Automated Segmentation of Organoid Images.

Maath Alani, Hamid A Jalab, Selin Pars, Bahaa Al-Mhanawi, Rowaida Z Taha, Ernst J Wolvetang, Mohammed R Shaker

Abstract read
In one paragraph

Article in Bioengineering (Basel, Switzerland), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Article
  3. Review
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Maath AlaniAustralian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, QLD 4072, Australia.
Hamid A JalabInformation and Communication Technology Research Group, Scientific Research Centre, Alayen University, Nasiriyah 64001, Thi Qar, Iraq.
Selin ParsAustralian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, QLD 4072, Australia.ORCID 0009-0003-9618-7956
Bahaa Al-MhanawiAustralian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, QLD 4072, Australia.ORCID 0000-0002-7425-3364
Rowaida Z TahaNeurological Disorders Research Center, Qatar Biomedical Research Institute, Hamad Bin Khalifa University, Qatar Foundation, Education City P.O. Box 34110, Doha, Qatar.ORCID 0000-0001-6119-8389
Ernst J WolvetangAustralian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, QLD 4072, Australia.ORCID 0000-0002-2146-6614
Mohammed R ShakerAustralian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, QLD 4072, Australia.ORCID 0000-0002-3675-4598

Funding

Australian National Health and Medical Research Council 2020434Australian National Health and Medical Research Council GA56111Australian National Health and Medical Research Council MRFF 2024380QBRI Interdisciplinary Research Program IDRP-2019-001QBRI Start-up Funding SF-2025_003
6 · The paper itself

Abstract

Organoids have emerged as powerful in vitro models for studying human development, disease mechanisms, and drug responses. A critical aspect of organoid characterisation is monitoring changes in size and morphology during culture; however, extracting these metrics from high-throughput imaging datasets is time-consuming and often inconsistent. Automated deep-learning approaches can overcome this bottleneck by providing accurate and reproducible image analysis. Here, we present an enhanced U-net-based segmentation model that incorporates region-of-interest refinement to improve the delineation of organoid boundaries. The method was validated on bright-field organoid images and demonstrated robust performance, achieving an accuracy of 98.15%, a dice similarity coefficient of 97.19%, and a Jaccard index of 94.53%. Compared with conventional segmentation methods, our model provides superior boundary detection and morphological quantification. These results highlight the potential of this approach as a reliable tool for high-throughput organoid analysis, supporting applications in disease modelling, drug screening, and personalised medicine.

Indexed as

convolutional neural networkdeep learningdice similarity coefficientimage segmentationJaccard indexorganoidsU-net

Identifiers

PMID41301171
PMCPMC12650739

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.