Evidence map›Paper›PMID 41303668›Full record

ArticleInternational journal of molecular sciences2025

Mapping the Nitric Oxide Axis in IVF: Genotype Associations in Antagonist Cycles.

Charalampos Voros, Fotios Chatzinikolaou, Georgios Papadimas, Spyridon Polykalas, Despoina Mavrogianni, Aristotelis-Marios Koulakmanidis, Diamantis Athanasiou, Vasiliki Kanaka, Maria Kanaka, Kyriakos Bananis and 10 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

20 authors.

Charalampos Voros1st Department of Obstetrics and Gynecology, 'Alexandra' General Hospital, National and Kapodistrian University of Athens, 80 Vasilissis Sofias Avenue, 11528 Athens, Greece.ORCID 0000-0001-9477-7619
Fotios ChatzinikolaouLaboratory of Forensic Medicine and Toxicology, School of Medicine, Aristotle University of Thessaloniki, 54124 Athens, Greece.
Georgios PapadimasAthens Medical School, National and Kapodistrian University of Athens, 15772 Athens, Greece.
Spyridon PolykalasAthens Medical School, National and Kapodistrian University of Athens, 15772 Athens, Greece.
Despoina Mavrogianni1st Department of Obstetrics and Gynecology, 'Alexandra' General Hospital, National and Kapodistrian University of Athens, 80 Vasilissis Sofias Avenue, 11528 Athens, Greece.ORCID 0000-0003-1220-6594
Aristotelis-Marios Koulakmanidis1st Department of Obstetrics and Gynecology, 'Alexandra' General Hospital, National and Kapodistrian University of Athens, 80 Vasilissis Sofias Avenue, 11528 Athens, Greece.
Diamantis AthanasiouIVF Athens Reproduction Center, 15123 Maroussi, Greece.
Vasiliki Kanaka1st Department of Obstetrics and Gynecology, 'Alexandra' General Hospital, National and Kapodistrian University of Athens, 80 Vasilissis Sofias Avenue, 11528 Athens, Greece.
Maria Kanaka1st Department of Obstetrics and Gynecology, 'Alexandra' General Hospital, National and Kapodistrian University of Athens, 80 Vasilissis Sofias Avenue, 11528 Athens, Greece.
Kyriakos BananisKing's College Hospitals NHS Foundation Trust, London SE5 9RS, UK.ORCID 0000-0001-7563-4305
Antonia AthanasiouIVF Athens Reproduction Center, 15123 Maroussi, Greece.
Aikaterini AthanasiouIVF Athens Reproduction Center, 15123 Maroussi, Greece.
Ioannis K PapapanagiotouAthens Medical School, National and Kapodistrian University of Athens, 15772 Athens, Greece.ORCID 0000-0002-9723-5054
Charalampos Tsimpoukelis1st Department of Obstetrics and Gynecology, 'Alexandra' General Hospital, National and Kapodistrian University of Athens, 80 Vasilissis Sofias Avenue, 11528 Athens, Greece.
Maria Anastasia Daskalaki1st Department of Obstetrics and Gynecology, 'Alexandra' General Hospital, National and Kapodistrian University of Athens, 80 Vasilissis Sofias Avenue, 11528 Athens, Greece.
Marianna Theodora1st Department of Obstetrics and Gynecology, 'Alexandra' General Hospital, National and Kapodistrian University of Athens, 80 Vasilissis Sofias Avenue, 11528 Athens, Greece.ORCID 0000-0001-8280-7796
Nikolaos Thomakos1st Department of Obstetrics and Gynecology, 'Alexandra' General Hospital, National and Kapodistrian University of Athens, 80 Vasilissis Sofias Avenue, 11528 Athens, Greece.
Panagiotis Antsaklis1st Department of Obstetrics and Gynecology, 'Alexandra' General Hospital, National and Kapodistrian University of Athens, 80 Vasilissis Sofias Avenue, 11528 Athens, Greece.ORCID 0000-0003-2106-5922
Georgios Daskalakis1st Department of Obstetrics and Gynecology, 'Alexandra' General Hospital, National and Kapodistrian University of Athens, 80 Vasilissis Sofias Avenue, 11528 Athens, Greece.ORCID 0000-0001-7108-211X
Dimitrios LoutradisLaboratory of Forensic Medicine and Toxicology, School of Medicine, Aristotle University of Thessaloniki, 54124 Athens, Greece.ORCID 0000-0002-8686-594X

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Endothelial nitric oxide synthase (eNOS, NOS3) regulates steroidogenesis, redox signalling, and the vascular tone of the ovaries. Despite varying outcomes in previous studies, the prevalent NOS3 rs1799983 (Glu298Asp) polymorphism may influence endocrine function during controlled ovarian stimulation (COS). On the retrieval day, we assessed follicular-fluid hormones, day-3 hormones, and controlled ovarian stimulation (COS) outcomes (follicles, oocytes, MII oocytes, embryos) in 62 antagonist IVF/ICSI cycles classified by NOS3 genotype (GG/GT/TT). The outcomes for COS and early-cycle hormones were mostly consistent across all genotypes. A similar allele-dose pattern was seen for baseline oestradiol (GG < GT < TT), with heterozygous carriers displaying higher levels of follicular-fluid β-hCG relative to GG individuals. No changes were seen in follicle count, oocyte production, nuclear maturation, or embryo development. Baseline oestradiol and follicular β-hCG serve as the principal indications of the modest, context-dependent endocrine effects of the NOS3 rs1799983 polymorphism in antagonist cycles. To clarify the clinical significance of these intricate genotype-associated patterns, additional comprehensive, genotype-balanced investigations that include direct NO-pathway phenotyping are essential.

Indexed as

Fertilization in VitroNitric OxideNitric Oxide Synthase Type IIIAdultEstradiolFemaleFollicular FluidGenotypeHumansOocytesOvarian FollicleOvulation InductionPolymorphism, Single NucleotideEstradiolNitric OxideNitric Oxide Synthase Type IIINOS3 protein, humanendothelial nitric oxide synthasefollicular fluidgonadotropin responseIVFnitric oxideNOS3 polymorphismoocyte competenceovarian stimulationrs1799983 (Glu298Asp)β-hCG

Identifiers

PMID41303668
PMCPMC12653611

What Socratic holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.