ReviewInternational journal of molecular sciences2025
Advances in Cytotoxicity Testing: From In Vitro Assays to In Silico Models.
Review in International journal of molecular sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed.
- Toxicity of nanostructures in drug delivery applications.Pharmaceutical science advances · 2026Review
- Pharmaceutical innovation in advanced nanomedicine for the treatment of hypervirulent and multidrug-resistant Klebsiella pneumoniae infections.Archives of microbiology · 2026Review
- Beyond Tuberculosis: Unlocking the Antifungal Potential of Novel Isoniazid Derivatives against Non-ACS omega · 2026Article
- Toward Dynamic Biomimetic Biomaterials: Temporal Coupling of Flavonoid-Induced Cellular Responses and 3D-Printed PLA Scaffold Evolution.Biomimetics (Basel, Switzerland) · 2026Article
- Toxicological Assessment of the Marine-Derived Cyclic Dipeptide Cyclo(Pro-Tyr) Using In Vitro, Zebrafish Embryo, and BALB/c Mouse Models.Journal of xenobiotics · 2026Article
- Current and emerging new approach & methodologies for skin hazard assessment.Toxicological research · 2026Review
- From Executor to Orchestrator: The Pharmacology Scientist in the Age of Agentic AI.Clinical pharmacology and therapeutics · 2026Review
- Comparative Physicochemical Characterization and Biological Effects of Magnetite- and Maghemite-Based Magnetic Colloidal Suspensions in A704 Renal Cancer Cells.Pharmaceuticals (Basel, Switzerland) · 2026Article
- Protein Modifications for Cellular Protein Delivery.Chemical reviews · 2026Review
- Plant-Derived Natural Products and Selective Apoptosis: A Cancer-Cell Vulnerability-State Framework from Redox Imbalance to Membrane-Ion Dysregulation.Pharmaceuticals (Basel, Switzerland) · 2026Review
- Integrated Phytochemical and Pharmacological Insights intoPlants (Basel, Switzerland) · 2026Review
- AdvancingBiology · 2026Article
- Cytotoxicity of Self-Etch Versus Etch-and-Rinse Dentin Adhesives After 48 h: An In Vitro Study.Dentistry journal · 2026Article
- Cytotoxicity of hexagonal boron nitride incorporated conventional and resin modified glass ionomer cements: in vitro study.BMC oral health · 2026Article
- A review of phytochemicals targeting bacterial biofilm and quorum sensing pathways: molecular, microbiological, and mechanistic insights.Archives of microbiology · 2026Review
- Cinnamon Bark Essential Oil as a Natural Plant Protection Agent: Chemical Profile, Antimicrobial Activity, and Defence Induction.Molecules (Basel, Switzerland) · 2026Article
- Evaluation of the Cytotoxic Effects of Adhesive Systems with Different pH Values on L929 Fibroblast Cells: An In Vitro Study.Bioengineering (Basel, Switzerland) · 2026Article
- New Derivatives of 4-Piperidinylphenyl-Linked Thiazoles as VEGFR2 Inhibitors with Potential Cytotoxicity Against Renal Cancer.Biomolecules · 2026Article
- Artificial Intelligence in the Design and Development of Nanoparticle Drug Delivery Systems: A Systematic Review.Advances in pharmacological and pharmaceutical sciences · 2026Review
- Robustness Analyses Substantially Narrow in silico Network-Toxicology Signals Linking Selected Pharmaceuticals and Caffeine to Vitiligo.Clinical, cosmetic and investigational dermatology · 2026Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
1 author.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cytotoxicity testing remains a cornerstone of modern toxicology, providing critical insight into how chemicals and drugs affect cell viability and function. Classical colorimetric assays such as MTT, LDH release, and neutral red uptake established the methodological basis of in vitro toxicology and continue to serve as regulatory benchmarks. However, their limited mechanistic depth and physiological relevance have prompted the field to evolve towards more predictive and human-centred approaches. Recent advances in high-content imaging, flow cytometry, and real-time impedance analysis have transformed cytotoxicity testing into a multiparametric discipline capable of detecting adaptive and sub-lethal cellular responses. Parallel progress in computational toxicology has introduced in silico models-QSAR, machine learning, and physiologically based pharmacokinetic (PBPK) modelling-that enable quantitative in vitro-in vivo extrapolation (QIVIVE). The integration of these computational tools with 3D organoids, organ-on-chip systems, and stem cell-based models allows for cross-validation between predictive simulations and experimental evidence, enhancing mechanistic interpretation and translational accuracy. Together, these developments underpin New Approach Methodologies (NAMs) and Integrated Approaches to Testing and Assessment (IATA), marking the transition from descriptive assays to predictive, mechanism-anchored frameworks that bridge in silico prediction with in vitro and in vivo validation-advancing both biomedical research and regulatory toxicology.
Indexed as
Identifiers
What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.