Evidence map›Paper›PMID 41309574›Full record

ArticleNature communications2025

The luminal domain region of Seipin/Fld1 is dispensable for establishing functional ER sites for lipid droplet biogenesis.

Amit Khatri, Ritika Chaudhary, R Mankamna Kumari, Vineet Choudhary

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Review
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Amit Khatri *Lipid Metabolism Laboratory, Department of Biotechnology, All India Institute of Medical Sciences (AIIMS), New Delhi, 110029, India.ORCID http://orcid.org/0009-0004-7514-7864
Ritika Chaudhary *Lipid Metabolism Laboratory, Department of Biotechnology, All India Institute of Medical Sciences (AIIMS), New Delhi, 110029, India.ORCID http://orcid.org/0009-0009-0260-1268
R Mankamna KumariLipid Metabolism Laboratory, Department of Biotechnology, All India Institute of Medical Sciences (AIIMS), New Delhi, 110029, India.
Vineet ChoudharyLipid Metabolism Laboratory, Department of Biotechnology, All India Institute of Medical Sciences (AIIMS), New Delhi, 110029, India. vchoudhary@aiims.edu.ORCID http://orcid.org/0000-0003-3327-676X

Funding

DBT India Alliance (Wellcome Trust/DBT India Alliance) IA/I/20/2/505191Department of Biotechnology, Ministry of Science and Technology (DBT) IC-12044(11)/6/2021-ICD- DBTWellcome Trust
6 · The paper itself

Abstract

Lipid droplet (LD) biogenesis occurs in the endoplasmic reticulum (ER), the mechanisms of which is not completely known. Seipin (Fld1 in yeast) is a crucial ER membrane protein that defines LD biogenesis sites. Here, we show that truncated seipin, Fld1-∆LR in yeast, and the human equivalent hSeipin-∆LR, mutants lacking the conserved luminal domain region (LR), functionally complement the LD biogenesis defect of fld1∆ mutants. Fld1-∆LR foci colocalize with factors: Nem1, Ldb16, Pex30 and Yft2, which are important for LD biogenesis and these sites become enriched in diacylglycerol upon stimulation of LD formation. Fld1-∆LR forms a homo-oligomeric complex facilitated by protein-protein interactions. We show that mutating the 31

Indexed as

Endoplasmic ReticulumGTP-Binding Protein beta SubunitsGTP-Binding Protein gamma SubunitsLipid DropletsSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsHumansMembrane ProteinsMitochondrial ProteinsNuclear ProteinsProtein DomainsTriglyceridesBSCL2 protein, humanFld1 protein, S cerevisiaeGTP-Binding Protein beta SubunitsGTP-Binding Protein gamma SubunitsLDB16 protein, S cerevisiaeMembrane ProteinsMitochondrial ProteinsNem1 protein, S cerevisiaeNuclear ProteinsSaccharomyces cerevisiae Proteinsseipin protein, S cerevisiaeTriglycerides

Identifiers

PMID41309574
PMCPMC12660851

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.