ArticleStem cell research & therapy2025
Synergistic effects of mesenchymal stem cell secretome and Wnt10b on hair follicle regeneration in a 3D bioengineering model: a preclinical study.
Article in Stem cell research & therapy, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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Who cites it
2 citing papers in PubMed.
- Organoid-loaded core-shell cryomicroneedles induce biomimetic follicular units and hair regeneration.Bioactive materials · 2026Article
- Stem cell-based approaches for alopecia: A narrative review of current and emerging treatment strategies.Cell transplantationReview
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Authors and funding
3 authors.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundDespite all advances, constructing functional hair follicles remains a significant clinical challenge. Building on recent advancements in mesenchymal stem cells secretome and Wnt signaling, we aimed to fabricate hair follicles in a 3D hydrogel scaffold and assess the effects of placenta-derived MSCs (PMSC) secretome and Wnt10b on follicle formation in ex vivo and in vivo models.
methodsA gelatin methacryloyl (GelMA) hydrogel scaffold was engineered via a 3D-printed We engineered a gelatin methacryloyl (GelMA) hydrogel scaffold using a 3D-printed polydimethylsiloxane mold to create a co-culture system for hair follicle biofabrication. Dermal papilla cells (DPCs) and epithelial cells from a donor were isolated and seeded into the scaffold to form microfollicles. We treated hair microfollicles with PMSC secretome, Wnt10b protein, or combination of both. The generated hair microfollicles were assessed using specific molecular and protein markers and then implanted into nude mice skin to investigate the hair growth in vivo.
resultsAfter three weeks of co-culturing, the group receiving both secretome and Wnt10b showed 90% cell aggregation and highest hair follicle formation rate at 28% (p < 0.0001). Immunofluorescent analysis revealed elevated expression levels of markers for DPCs and epithelial cells in formed hair follicles. Real-time PCR analysis indicated significantly enhanced expression of Wnt10b, Bmp6, Notch1 and Notch2 genes, particularly in the group receiving secretome and Wnt10b. Transplantation of GelMA hydrogels containing hair microfollicles into nude mice resulted in successful hair formation along with immunofluorescence and gene expression analyses showing marked increases in hair follicle growth markers.
conclusionOur study demonstrates that the combination of PMSC-secretome and Wnt10b in a GelMA hydrogel scaffold effectively promotes hair follicle biofabrication and successful hair formation in vivo. However, the in vivo portion was limited by a small sample size (n = 3 per group), underscoring the need for larger-scale studies to confirm these findings.
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