ArticleFrontiers in microbiology2025
Evaluation of serological assays for
Article in Frontiers in microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
3 citing papers in PubMed.
- A comprehensive review of bacterial and hemoparasitic diseases in the water buffalo.Frontiers in veterinary science · 2026Review
- Evaluation of brucellosis eradication strategies in water buffalo in a key dairy production area of southern Italy.Frontiers in microbiology · 2026Article
- Exploring alternative cytokines as potential biomarkers forFrontiers in immunology · 2026Article
Corrections and comments
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Authors and funding
12 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Introduction: Bovine tuberculosis (TB) in water buffalo ( Methods: This study analyzed 895 serum samples from water buffaloes, including 393 from TB-free herds and 502 from TB-infected herds. Animals from TB-free herds were tested using IDT and an ELISA assay, whereas those from infected herds were also tested using IGRA. We developed an ELISA assay targeting MPB70, MPB83, ESAT6, CFP10, PPDB, and P22 antigens to investigate the role of the humoral response in TB diagnosis. Results and discussion: The ELISA showed a specificity of 98.2%. However, sensitivity differed based on the antigen used: among the most reactive proteins, sensitivity was 67.5% for MPB70, 69.8% for P22, and 74.4% for PPDB. Moreover, approximately 70% of samples with discordant IDT and IGRA results, as well as those with positive IDT but inconclusive IGRA results, tested positive by serology, highlighting the potential of antibody-based detection to improve TB diagnosis in buffaloes. Our findings suggest that integrating serological testing with standard diagnostic methods could enhance the detection of infected animals, ultimately contributing to better TB control in buffalo populations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.