Evidence map›Paper›PMID 41311899›Full record

ArticleChemical & biomedical imaging2025

Wide-Field Digital Surface-Enhanced Raman Scattering: Quantitative Single-Molecule Detection with High Sensitivity and Throughput.

Siyang Ye, Wen Zhang, Ling Tang, Kuanyu Ma, Jinling Ma, Li Li, Weigao Xu, Zhonghua Xi, Yuxi Tian

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Chemical & biomedical imaging, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Siyang YeState Key Laboratory of Analytical Chemistry for Life Science, Key Laboratory of Mesoscopic Chemistry of MOE and School of Chemistry and Chemical Engineering, Nanjing University, 210023 Nanjing, China.ORCID https://orcid.org/0009-0001-4973-5476
Wen ZhangState Key Laboratory of Analytical Chemistry for Life Science, Key Laboratory of Mesoscopic Chemistry of MOE and School of Chemistry and Chemical Engineering, Nanjing University, 210023 Nanjing, China.
Ling TangState Key Laboratory of Analytical Chemistry for Life Science, Key Laboratory of Mesoscopic Chemistry of MOE and School of Chemistry and Chemical Engineering, Nanjing University, 210023 Nanjing, China.
Kuanyu MaState Key Laboratory of Analytical Chemistry for Life Science, Key Laboratory of Mesoscopic Chemistry of MOE and School of Chemistry and Chemical Engineering, Nanjing University, 210023 Nanjing, China.
Jinling MaState Key Laboratory of Analytical Chemistry for Life Science, Key Laboratory of Mesoscopic Chemistry of MOE and School of Chemistry and Chemical Engineering, Nanjing University, 210023 Nanjing, China.
Li LiState Key Laboratory of Coordination Chemistry, Key Laboratory of Mesoscopic Chemistry of MOE, School of Chemistry and Chemical Engineering, Nanjing University, 210023 Nanjing, China.
Weigao XuState Key Laboratory of Coordination Chemistry, Key Laboratory of Mesoscopic Chemistry of MOE, School of Chemistry and Chemical Engineering, Nanjing University, 210023 Nanjing, China.ORCID https://orcid.org/0000-0002-3014-756X
Zhonghua XiState Key Laboratory of Analytical Chemistry for Life Science, Key Laboratory of Mesoscopic Chemistry of MOE and School of Chemistry and Chemical Engineering, Nanjing University, 210023 Nanjing, China.
Yuxi TianState Key Laboratory of Analytical Chemistry for Life Science, Key Laboratory of Mesoscopic Chemistry of MOE and School of Chemistry and Chemical Engineering, Nanjing University, 210023 Nanjing, China.ORCID https://orcid.org/0000-0002-5910-1514

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Due to its exceptionally high sensitivity and specificity, surface-enhanced Raman scattering (SERS) is widely employed in diverse fields, including biomedicine, environmental monitoring, and food safety. Nonetheless, the lack of reproducibility and substrate uniformity has seriously hindered its application in quantitative detection, particularly at low analyte concentrations. Recently, the concept of digitization has been integrated into SERS, enabling quantitative and sensitive detection with promising applications (Bi et al. Nature2024, 628, 771-775). In this work, we further developed a wide-field digital SERS (WidiSERS) by employing wide-field microscopy for high throughput. Protein assembled gold nanorod dimers are used for largely enhancing the Raman signals. Reproducible quantification of a wide range of target molecules at extremely low concentrations is achievable through single-molecule measurements. Trace-level quantification of ciprofloxacin in a complex milk environment and phenylalanine in cell culture medium was also achieved, verifying the practicability and accuracy of this method. Meanwhile, the gold nanorod dimer substrate is both simple to prepare and reusable after UV/ozone cleaning. WidiSERS is expected to emerge as a preferred method for ultrafast and effective detection across various fields.

Indexed as

digital SERSgold nanorodquantitativeRamanSingle moleculewide field imaging

Identifiers

PMID41311899
PMCPMC12648421

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.