Evidence mapPaperPMID 41314281Full record

ArticleJournal of advanced research2026

Selenoprotein H targets MTCH2 to regulate MFN2-dependent mitochondrial quality control to alleviate acute kidney injury.

Dongliu Luo, Yaning Qiu, Jiahong Chu, Haodong Hu, Yanhe Zhang, Yu Xia, Fuze She, Shiwen Xu, Fating Zhu, Zhiruo Miao and 1 more

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Article in Journal of advanced research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

11 authors.

Dongliu LuoCollege of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China; College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471023, China.
Yaning QiuCollege of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China.
Jiahong ChuCollege of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China.
Haodong HuCollege of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China.
Yanhe ZhangCollege of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China.
Yu XiaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China; Animal Science College, Hebei North University, Zhangjiakou 075000, China.
Fuze SheCollege of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China.
Shiwen XuCollege of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China.
Fating ZhuNational Selenium-Rich Product Quality Supervision and Inspection Center, Enshi 445000, China.
Zhiruo MiaoCollege of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China. Electronic address: zhiruomiao@neau.edu.cn.
Shu LiCollege of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China. Electronic address: lishu@neau.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionMitochondrial dysfunction is recognized as a pivotal event in the pathogenesis of acute kidney injury (AKI). Selenoprotein (SelH), a mammalian selenoprotein, is extensively involved in regulating of diseases associated with mitochondrial dysfunction. However, its regulatory role in mitochondrial quality control during AKI remains unclear.

objectivesThis study aims to explore the impact of SelH on AKI and potential regulatory mechanisms of SelH in AKI.

methodsIn vivo, a cisplatin (CP)-induced AKI model was established using SelH knockout mice to evaluate renal injury. Additionally, co-immunoprecipitation (Co-IP). combined with mass spectrometry, Co-IP assays, laser confocal microscopy, and molecular docking were employed to identify proteins interacting with SelH. In vitro, SelH/mitochondrial carrier homolog 2 (MTCH2) knockdown and overexpression models were constructed in HEK293t cells. Indicators related to oxidative stress, mitochondrial biogenesis, mitochondrial dynamics, mitophagy, and apoptosis were analyzed.

resultsMTCH2 was identified as a potential interacting partner of SelH. Deficiency of renal SelH directly triggered oxidative stress, impaired mitochondrial biogenesis, disrupted mitochondrial dynamics, enhanced mitophagy, and promoted apoptosis. In HEK293t cells, SelH targeted MTCH2 to regulate mitofusin 2 (MFN2), thereby promoting mitochondrial fusion, alleviating mitochondrial dysfunction, maintaining mitochondrial quality control (MQC) homeostasis, and reducing renal oxidative damage and apoptosis.

conclusionThe results showed that SelH targets the MTCH2/MFN2 aixs to maintain MQC balance, alleviate oxidative stress and cell apoptosis induced by AKI. This study not only supplements kidney specific regulatory targets for the field of mitochondrial medicine but also suggests that SelH could serve as a potential molecule for proactive medicine intervention in AKI, providing experimental evidence for the early intervention of AKI.

Indexed as

Acute Kidney InjuryGTP PhosphohydrolasesMitochondriaMitochondrial Membrane Transport ProteinsSelenoproteinsAnimalsApoptosisCisplatinDisease Models, AnimalHEK293 CellsHumansMaleMiceMice, KnockoutMitochondrial DynamicsMitochondrial ProteinsCisplatinGTP PhosphohydrolasesMFN2 protein, humanMfn2 protein, mouseMitochondrial Membrane Transport ProteinsMitochondrial ProteinsSelenoproteinsAcute kidney injuryApoptosisMTCH2Restored mitochondrial homeostasis and functionalitySelenoprotein H

Identifiers

PMID41314281
PMCPMC13453858

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.