Evidence map›Paper›PMID 41315141›Full record

ArticleMolecular neurobiology2025

Black Soybean Peptides Attenuate Lead-Induced Neurotoxicity: Role of Oxidative Stress and the RhoA/MAPK Signaling Pathway.

Huijie Yang, Fangyu Wang, Qian Li, Tiange Li, Bei Zhang, Lianjun Song, Xianqing Huang, Shuyang Liu, Gianni Galaverna, Peijun Zhao and 2 more

Abstract read
PubMed Publisher
In one paragraph

Article in Molecular neurobiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Huijie YangCollege of Food Science and Technology, Henan Agricultural University, 63#Agricultural Road, Zhengzhou, 450000, China.
Fangyu WangKey Laboratory for Animal Immunology, Henan Academy of Agricultural Sciences, 116#Huayuan Road, Zhengzhou, 450002, China.
Qian LiCollege of Food Science and Technology, Henan Agricultural University, 63#Agricultural Road, Zhengzhou, 450000, China.
Tiange LiCollege of Food Science and Technology, Henan Agricultural University, 63#Agricultural Road, Zhengzhou, 450000, China.
Bei ZhangCollege of Food Science and Technology, Henan Agricultural University, 63#Agricultural Road, Zhengzhou, 450000, China.
Lianjun SongCollege of Food Science and Technology, Henan Agricultural University, 63#Agricultural Road, Zhengzhou, 450000, China.
Xianqing HuangCollege of Food Science and Technology, Henan Agricultural University, 63#Agricultural Road, Zhengzhou, 450000, China.
Shuyang LiuCollege of Food Science and Technology, Henan Agricultural University, 63#Agricultural Road, Zhengzhou, 450000, China.
Gianni GalavernaFood and Drug Department, University of Parma, Parco Area Delle Scienze, 17/a, Parma, 43124, Italy.
Peijun ZhaoCollege of Food Science and Technology, Henan Agricultural University, 63#Agricultural Road, Zhengzhou, 450000, China. zhaopeijun@henau.edu.cn.
Ibrahim A BakryCollege of Food Science and Technology, Henan Agricultural University, 63#Agricultural Road, Zhengzhou, 450000, China.
Ning LiCollege of Food Science and Technology, Henan Agricultural University, 63#Agricultural Road, Zhengzhou, 450000, China. ningli@henau.edu.cn.

Funding

National Natural Science Foundation of China NNSFC-31201878, U1204804, and U1604183
6 · The paper itself

Abstract

Lead (Pb) exposure is a major environmental risk factor for neurodegenerative diseases. This study investigates the neuroprotective effects of black soybean peptides (BSPs), particularly BSP1, BSP3, and BSP4, against Pb-induced toxicity in HT22 mouse hippocampal neuron cells, with a mechanistic focus on the RhoA/MAPK signaling pathway. The rationale for targeting RhoA/MAPK stems from their established roles in mediating oxidative stress and apoptosis in Pb-related pathology. Pretreatment with BSP1 or BSP4 (200 µM) increased cell viability by approximately 35% relative to the Pb-only group. At the same time, BSPs also decreased intracellular reactive oxygen species (ROS) levels by up to 40% and malondialdehyde (MDA) by 30%. Antioxidant enzyme activities, including superoxide dismutase (SOD) and catalase (CAT), were restored to near-control levels; for example, SOD activity increased 1.8-fold compared with the Pb group. Western blot and immunofluorescence confirmed that BSPs reduced the Pb-triggered activation of RhoA, ROCK1/2, and MAPK proteins (p38, JNK, ERK). These findings demonstrate that BSPs mitigate Pb-induced neurotoxicity by enhancing antioxidant activity and targeting RhoA/MAPK signaling, highlighting their potential as functional food ingredients or therapeutic agents for neuroprotection.

Indexed as

Glycine maxLeadMAP Kinase Signaling SystemOxidative StressPeptidesrhoA GTP-Binding ProteinAnimalsAntioxidantsCell LineCell SurvivalHippocampusMiceNeuronsNeuroprotective AgentsReactive Oxygen SpeciesSuperoxide DismutaseAntioxidantsLeadNeuroprotective AgentsPeptidesReactive Oxygen SpeciesrhoA GTP-Binding ProteinSuperoxide DismutaseBlack soybean peptidesLead toxicityNeuroprotectionOxidative stressRhoA/MAPK pathway

Identifiers

PMID41315141

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.