ArticleNature communications2025
An apical ring protein essential for conoid complex assembly and daughter cell formation in Toxoplasma gondii.
Article in Nature communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Dual apical methyltransferases orchestrate motility initiation in apicomplexan parasites.Nature communications · 2026Article
- The apical polar ring is essential during the blood stage of Plasmodium falciparum.Nature microbiology · 2026Article
- The human parasite, Toxoplasma gondii, is paralyzed without two components of the apical polar ring.PLoS pathogens · 2026Article
- The cytoskeleton of pathogenic protists.Bioscience reports · 2026Review
- Two anchoring proteins control daughter apical complex assembly inbioRxiv : the preprint server for biology · 2026Article
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Authors and funding
10 authors.
Funding
Abstract
In Toxoplasma gondii, the conoid complex consists of intraconoidal microtubules (ICMTs), preconoidal rings (PCRs), apical polar ring (APR), and the conoid. This organelle plays an important role for initiation of gliding motility, required for host cell invasion and egress. The molecular mechanisms governing stepwise assembly of the conoid complex remain poorly understood. We previously identified CGP, an essential protein required for motility initiation. Here, we demonstrate that CGP is crucial for anchoring FRM1 and other PCR components to mature PCRs, while the initial assembly in daughter cells is unaffected. Cryo-electron tomography of CGP-depleted parasites reveals the absence of the PCRs in the mature parasites, demonstrating that CGP is essential for stabilising the PCRs after replication. Using CGP as bait, we identify a protein required for the early assembly of the nascent conoid complex. The APR scaffold assembly factor (ASAF1) defines the position of the conoid complex before tubulin polymerisation. Depletion of ASAF1 results in failure of conoid complex assembly, disorganised microtubules, and lack of daughter cell formation. Collectively, our findings reveal two essential proteins that play critical roles in the early and late stages of conoid complex formation, providing insight into the mechanisms of conoid complex assembly.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.