Observational studyScientific reports2025
Quantitative assessment of the corneal subbasal nerve plexus in children with neurofibromatosis type 1 and optic pathway glioma using in vivo confocal microscopy.
Observational study in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To quantitatively evaluate corneal subbasal nerve plexus (SNP) in children with neurofibromatosis type 1 (NF1) and optic pathway glioma using in vivo confocal microscopy (IVCM), comparing semi-automated and automated image analysis methods. In this prospective observational case series, 13 eyes of 7 children with NF1 and optic pathway glioma and 10 eyes of 6 age-matched healthy controls underwent IVCM imaging. Three high-quality central corneal images per eye were analyzed using NeuronJ (semi-automated) and ACCMetrics (automated) software. Corneal nerve fiber length (CNFL), nerve fiber density (CNFD), branch density, fiber area, fiber width, and fractal dimension were quantified. Group comparisons used t-tests and linear mixed modeling, accounting for inter-eye correlation. Agreement between methods was assessed with Bland-Altman analysis. NF1 eyes showed significantly greater CNFL on NeuronJ (27.13 ± 5.17 vs. 22.70 ± 4.98 mm/mm²; P = 0.042) and higher CNFD on ACCMetrics (27.32 ± 10.64 vs. 20.83 ± 7.98 fibers/mm²; P = 0.038) compared with controls. Other ACCMetrics parameters (CNFL, CNBD, CTBD, CNFA, CNFW, CNFrD) were not significantly different. Bland-Altman analysis demonstrated poor agreement between methods, with ACCMetrics systematically underestimating CNFL relative to NeuronJ (bias = 8.85 mm/mm²; limits of agreement 2.20 to 15.50 mm/mm²) and increasing underestimation at higher CNFL values (r = 0.43, P < 0.001). Children with NF1 and optic pathway glioma exhibit increased corneal nerve fiber length and density, suggesting enhanced nerve proliferation or branching rather than hypertrophy. However, automated ACCMetrics underestimated CNFL relative to semi-automated NeuronJ, emphasizing the need for analytic standardization. Larger, longitudinal studies are required to confirm these findings and explore their potential as noninvasive biomarkers of NF1-associated neuropathy.
Indexed as
Identifiers
What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.