ArticleBMC plant biology2025
Genome-wide analysis of DGAT gene family in Coix lacryma jobi L. and functional characterization in yeast H1246.
Article in BMC plant biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
backgroundCoix lacryma jobi L., a member of the Poaceae family, is a traditional Chinese medicine ingredient with a long history. In recent years, research and clinical treatment have shown that Coix lacryma jobi L. seed oil exhibits significant anti-cancer effects and enhances the efficacy of chemotherapy. As Diacylglycerol Acyltransferase is a key rate-limiting enzyme in lipid synthesis, a thorough understanding of the Diacylglycerol Acyltransferase gene family in Coix lacryma jobi L. could pave the way for increasing its lipid content.
resultsThis study used the website NCBI to search for the CDS sequences of the Diacylglycerol Acyltransferase family genes in different plants and find the homologous genes in Coix lacryma jobi L. genome by blast screening. A total of ten Diacylglycerol Acyltransferase family genes were identified in Coix lacryma jobi L., which were named ClDGAT1_1, ClDGAT1_2, ClDGAT1_3, ClDGAT2_1, ClDGAT2-2, ClDGAT3, ClWS/DGAT_1, ClWS/DGAT_2, ClWS/DGAT_3, and ClWS/DGAT_4. We analyzed the protein physicochemical properties, gene structure, gene homology and evolutionary analysis of them to elaborate the information of ClDGAT family genes. Meanwhile, functional assays revealed significant differences in oil and fatty acid synthesis among the ClDGATs. By expressing ten ClDGAT genes in H1246 yeast and comparing the differences in oil and fatty acid content in these yeasts, we found that ClDGAT3 and ClDGAT1_2 had the best oil and triglyceride synthesis ability. This study advanced research on the Diacylglycerol Acyltransferase gene family and expanded the understanding of lipid synthesis-related genes in Coix lacryma jobi L..
conclusionsIn this study, we systematically identified and characterized ten DGAT family genes in the Coix lacryma jobi L. genome. Functional validation in the H1246 yeast demonstrated significant divergence in lipid synthesis capacity of ClDGAT isoforms. And ClDGAT3 and ClDGAT1_2 had the best oil and triglyceride synthesis ability.
Indexed as
Identifiers
What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.