Evidence map›Paper›PMID 41316370›Full record

ArticleJournal of ovarian research2025

Oviductus Ranae alleviates D-galactose-induced ovarian aging by inhibiting ferroptosis and regulating the GPX4/ACSL4 pathway.

Xiaomei Ling, Cong Xie, Ming Li, Jie Li, Shun Liu, Liyue Sun, Xinxin Zeng, Zhen Zhang, Xuhui Zhang, Lei Liang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of ovarian research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Xiaomei Ling *Medical Research Center, The Affiliated Guangdong Second Provincial General Hospital of Jinan University, Guangzhou, 510317, PR China.
Cong Xie *Department of Pediatrics, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510260, PR China.
Ming Li *Medical Research Center, The Affiliated Guangdong Second Provincial General Hospital of Jinan University, Guangzhou, 510317, PR China.
Jie LiMedical Research Center, The Affiliated Guangdong Second Provincial General Hospital of Jinan University, Guangzhou, 510317, PR China.
Shun LiuMedical Research Center, The Affiliated Guangdong Second Provincial General Hospital of Jinan University, Guangzhou, 510317, PR China.
Liyue SunDepartment of Health Management Centre, Zhongshan Hospital, Fudan University, Shanghai, 200032, PR China.
Xinxin ZengDepartment of Oncology, The Affiliated Guangdong Second Provincial General Hospital of Jinan University, Guangzhou, 510317, PR China.
Zhen ZhangMedical Research Center, The Affiliated Guangdong Second Provincial General Hospital of Jinan University, Guangzhou, 510317, PR China.
Xuhui ZhangDepartment of Oncology, The Affiliated Guangdong Second Provincial General Hospital of Jinan University, Guangzhou, 510317, PR China. zhangxh@gd2h.org.cn.
Lei LiangGuangdong Engineering Research Center of Chinese Medicine & Disease Susceptibility/ School of Traditional Chinese Medicine, Jinan University, Guangzhou, 510632, PR China. lhq_pharm@hotmail.com.

Funding

Foundation of Guangdong Second Provincial General Hospital 3DA2021015Foundation of Guangdong Second Provincial General Hospital YQ2020-004Guangdong-Hong Kong-Macao Universities Joint Laboratory for the Internationalization of Traditional Chinese Medicine 2023LSYS002Guangzhou Science and Technology Plan Projects 202201020247Guangzhou Science and Technology Plan Projects 202201020483National Natural Science Foundation of China 82374327Traditional Chinese Medicine Bureau of Guangdong Province 20222007
6 · The paper itself

Abstract

backgroundOvarian dysfunction caused by aging restricts female reproductive capacity and causes age-related health problems. Ferroptosis is an important mode of cell death during accelerated aging. Oviductus Ranae (OR), a traditional Chinese medicine, has been used to treat ovarian age-related diseases in women. However, the mechanisms through which OR mitigates ovarian aging, especially ferroptosis regulation, remain unclear. This study investigated the pharmacological effects and mechanisms of OR for ovarian aging in rats.

methodsSprague-Dawley female rats were divided into six groups: aging group induced with D-galactose; experiment groups treated with OR at low, middle, and high concentrations; positive control treated with estradiol valerate; and control group with no treatment. After 42 d, ovarian tissue and serum were collected for biochemical determination, hematoxylin and eosin staining, immunohistochemistry, enzyme-linked immunosorbent assay, reverse transcription-quantitative polymerase chain reaction, western blotting, and RNA sequencing analysis. To elucidate the mechanism underlying the effects of OR on ferroptosis in ovarian granulosa cells (OGCs), in vitro experiments were conducted.

resultsTreatment with OR improved ovarian follicular development and serum hormone levels, reduced iron deposition, enhanced cell proliferation, and inhibited apoptosis in D-galactose-induced ovarian aging rats. Ferroptosis was alleviated in the ovaries and serum by elevated glutathione and decreased lipid peroxidation production. RNA-seq showed that OR induced changes in 2,768 genes that were involved in ovarian steroidogenesis, glutathione metabolism, lipid metabolism, and ferroptosis pathways. Notably, OR downregulated ACSL4, LPCAT3, and ALOX12 mRNA and protein expressions, while upregulating FTH1, FTL, SLC7A11, GSS, and GPX4, which serve as critical regulators in ferroptosis. OGCs pretreated with OR-containing serum before erastin exposure exhibited enhanced viability, reduced ferrous iron, total iron, and lipid peroxidation levels, improved antioxidant activity, and stabilized mitochondrial function, indicating effective ferroptosis inhibition. Furthermore, OR-containing serum pretreatment inhibited ferroptosis in OGCs by regulating the GPX4/ACSL4 axis.

conclusionsOR resists ovarian damage and prevents aging through antiferroptosis mechanisms by regulating the GPX4/ACSL4 axis in OGCs and aging rats. These results highlight the potential of OR as a therapeutic agent for the prevention and treatment of ovarian aging.

Indexed as

AgingCoenzyme A LigasesDrugs, Chinese HerbalFerroptosisGalactoseOvaryPhospholipid Hydroperoxide Glutathione PeroxidaseAnimalsFemaleGranulosa CellsRatsRats, Sprague-DawleySignal TransductionCoenzyme A LigasesDrugs, Chinese HerbalGalactoseglutathione peroxidase 4, ratPhospholipid Hydroperoxide Glutathione PeroxidaseFerroptosisLipid peroxidationOvarian agingOvarian granulosa cellOviductus Ranae

Identifiers

PMID41316370
PMCPMC12781486

What Socratic holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.