Evidence map›Paper›PMID 41322359›Full record

ArticleFood chemistry. Molecular sciences2025

Adeno-associated virus-miR-128-3p sponge regulates breast muscle growth and intramuscular fat content in chickens.

Tingqi Zhu, Lekun Deng, Yuehua He, Wenjie Liang, Pingquan Liu, Shuangxing Liu, Haishan Guo, Donghua Li, Fengbin Yan, Yadong Tian and 6 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Food chemistry. Molecular sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Tingqi ZhuCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China.
Lekun DengCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China.
Yuehua HeCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China.
Wenjie LiangCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China.
Pingquan LiuCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China.
Shuangxing LiuCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China.
Haishan GuoCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China.
Donghua LiCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China.
Fengbin YanCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China.
Yadong TianCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China.
Ruirui JiangCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China.
Xiangtao KangCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China.
Weiwei JinCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China.
Zhunan LiCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China.
Wenting LiCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China.
Guirong SunCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Chicken meat is a major source of protein worldwide, and consumer demand for its consumption and quality is increasing. Although broilers grow rapidly to meet consumer demand, broilers have problems of low flavor substances and low intramuscular fat. Balancing the "quantity" and "quality" of chicken meat is the core of breeding. Among microRNAs, miR-128 can regulate myoblast proliferation and fat accumulation, it remains unclear whether miR-128 exerts the same function in vivo. Thus, this study constructed an adeno-associated virus (AAV)-mediated miR-128-3p sponge vector was constructed, and 20 chickens were equally assigned to a control group (injected with Cytomegalovirus (CMV) vector) and an experimental group (injected with AAV-miR-128-3p sponge vector). The results showed that the body weight, breast muscle weight, and breast muscle rate of the experimental group increased, the muscle fiber diameter enlarged, the mRNA expression of miR-128-3p in breast muscle decreased, and the lipid droplet area in breast and leg muscles as well as the crude fat content in breast muscle increased. In addition, lipidomic analysis revealed that the levels of 5 types of triglycerides (TG) in breast muscle was up-regulated. This study demonstrates that the AAV-miR-128-3p sponge vector can increase the growth rate and intramuscular fat (IMF) content of chickens, providing a targeted approach to improve poultry meat quality.

Indexed as

Chicken meatIntramuscular fatLipidomic analysisTriglycerides

Identifiers

PMID41322359
PMCPMC12657813

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.