Evidence mapPaperPMID 41322390Full record

ReviewBioImpacts : BI2025

Genetic engineering frontiers in cell manipulation-based tissue engineering: A comprehensive review.

Amirhossein Mohammadi, Mohammad Ali Heydari, Zahra Jamalpoor

Abstract readReview
In one paragraph

Review in BioImpacts : BI, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Review
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Amirhossein MohammadiTrauma and Surgery Research Center, Aja University of Medical Sciences, Tehran, Iran.ORCID https://orcid.org/0009-0001-0326-6352
Mohammad Ali HeydariTrauma and Surgery Research Center, Aja University of Medical Sciences, Tehran, Iran.
Zahra JamalpoorTrauma and Surgery Research Center, Aja University of Medical Sciences, Tehran, Iran.ORCID https://orcid.org/0000-0003-3598-5172

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: A new era of regenerative medicine has been ushered in by the combination of tissue engineering and genetic engineering, offering unprecedented opportunities to address the growing demand for functional tissue replacements. This narrative review explores cutting-edge approaches in cell manipulation-based tissue engineering through the lens of genetic engineering, highlighting the transformative potential of this synergy. Methods: We critically examine the application of advanced genetic engineering techniques, including CRISPR-Cas9, TALENs, and synthetic biology, in modifying cellular behaviors and functions for tissue engineering. The review encompasses a diverse range of engineered tissues, from cartilage and bone to cardiac, neural, skin, and vascular constructs, elucidating how genetic manipulation enhances their functionality and physiological relevance. We further investigate the integration of these genetic approaches with emerging technologies such as 3D-bioprinting, microfluidics, and smart biomaterials, which collectively expand the horizons of complex tissue fabrication. Results: The review delves into pioneering trends, including in vivo genetic engineering for tissue regeneration and the development of patient-specific engineered tissues, discussing their implications for personalized medicine. We address the field's challenges, including long-term genetic stability, scalability, and off-target effects, while also considering the ethical implications and evolving regulatory landscape of genetically engineered tissues. Emerging technologies in genetic engineering, including base editing and synthetic genetic circuits, have been explored for their potential to create "smart" tissues capable of dynamic environmental responses. The review also highlights the synergistic potential of combining genetic engineering with stem cell technologies to enhance tissue functionality and immunological compatibility. Conclusion: This comprehensive review concludes by underscoring the transformative impact of genetic engineering on cell manipulation-based tissue engineering. While significant challenges persist, the rapid advancements in this field herald a future where genetically tailored, functional tissue constructs could revolutionize regenerative medicine, offering new hope for addressing critical unmet medical needs.

Indexed as

BioengineeringCRISPRGene editingOrganoidsPersonalizationRegenerationSynthetic biology

Identifiers

PMID41322390
PMCPMC12663751

What Socratic holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.