Evidence map›Paper›PMID 41324865›Full record

ArticleMolecular and cellular biochemistry2026

FABP3 deficiency exacerbates renal Randall plaque formation: insights from single-cell RNA transcriptomic analysis.

Muhammad Sarfaraz Iqbal, Xiaolu Duan, Kotb A Attia, Ghulam Nabi, Kaoqing Peng, Nimra Sardar, Di Gu, Guohua Zeng, Nada S Al-Theyab

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Article in Molecular and cellular biochemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

9 authors.

Muhammad Sarfaraz IqbalDepartment of Urology, Minimally Invasive Surgery Center, Guangdong Key Laboratory of Urology, Guangzhou Urology Research Institute, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
Xiaolu DuanDepartment of Urology, Minimally Invasive Surgery Center, Guangdong Key Laboratory of Urology, Guangzhou Urology Research Institute, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
Kotb A AttiaCenter of Excellence in Biotechnology Research, King Saud University, Riyadh, 11451, Saudi Arabia.
Ghulam NabiSchool of Food Sciences and Biological Engineering, Jiangsu University, Zhenjiang, 212013, China.
Kaoqing PengDepartment of Urology, Minimally Invasive Surgery Center, Guangdong Key Laboratory of Urology, Guangzhou Urology Research Institute, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
Nimra SardarDepartment of Microbial Engineering, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China.
Di GuDepartment of Urology, Minimally Invasive Surgery Center, Guangdong Key Laboratory of Urology, Guangzhou Urology Research Institute, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
Guohua ZengDepartment of Urology, Minimally Invasive Surgery Center, Guangdong Key Laboratory of Urology, Guangzhou Urology Research Institute, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China. gzgyzgh@vip.sina.com.
Nada S Al-TheyabDepartment of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, Riyadh, 11451, Saudi Arabia. naaltheyab@ksu.edu.sa.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Randall’s plaque (RP) formation is a critical initiating process in calcium oxalate nephrolithiasis; however, the molecular mechanisms driving its pathogenesis remain incompletely understood. This study aimed to evaluate the role of fatty acid-binding protein 3 (FABP3), a key regulator of lipid metabolism and epithelial homeostasis, in RP development. We employed an integrative multi-omics approach incorporating single-cell RNA sequencing (scRNA-seq), quantitative real-time PCR (qPCR), immunohistochemistry (IHC), and pathway enrichment analyses on human renal papillary tissues from individuals with and without RP, alongside validation in a murine model. scRNA-seq revealed significant downregulation of FABP3 across 21 renal cell clusters within the RP microenvironment (mean expression: 0.0188). qPCR confirmed a tenfold reduction in FABP3 transcript levels in RP samples compared to controls (2^-ΔCt: 0.0714 vs. 0.6998; Cp values 24.05–25.31 vs. 20.97–22.77; p < 0.001), while IHC demonstrated parallel protein depletion in murine RP tissues. Functional enrichment analyses linked FABP3 deficiency to dysregulation of lipid metabolic pathways, including fatty acid transport and lipolysis, implicating these alterations in interstitial calcium deposition and plaque formation. In conclusion, this study identifies FABP3 as a central molecular determinant in RP pathogenesis, offering novel mechanistic insight and establishing FABP3 as a promising biomarker and therapeutic target for nephrolithiasis prevention.

Indexed as

Fatty Acid Binding Protein 3NephrolithiasisTranscriptomeAnimalsFemaleGene Expression ProfilingHumansMaleMiceSingle-Cell Gene Expression AnalysisFABP3 protein, humanFabp3 protein, mouseFatty Acid Binding Protein 3Differentially expressed genes (DEGs)Fatty acid-binding protein 3 (FABP3)Randall's plaque (RP)Single-cell RNA sequencing (scRNA-seq)

Identifiers

PMID41324865

What Socratic holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.