Characterization of a synthetic infectious cloned DNA of simian foamy virus serotype 1.

Sandra M Fuentes, Nicholas Mattson, Trent J Bosma, Eunhae H Bae, Arifa S Khan

Abstract read

In one paragraph

Article in Npj viruses, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Sandra M FuentesLaboratory of Retroviruses, Division of Viral Products, Office of Vaccines Research and Review, Center for Biologics Evaluation and Research, U.S. Food and Drug Administration, Silver Spring, MD, USA.

Nicholas MattsonLaboratory of Retroviruses, Division of Viral Products, Office of Vaccines Research and Review, Center for Biologics Evaluation and Research, U.S. Food and Drug Administration, Silver Spring, MD, USA.

Trent J BosmaLaboratory of Retroviruses, Division of Viral Products, Office of Vaccines Research and Review, Center for Biologics Evaluation and Research, U.S. Food and Drug Administration, Silver Spring, MD, USA.

Eunhae H BaeLaboratory of Retroviruses, Division of Viral Products, Office of Vaccines Research and Review, Center for Biologics Evaluation and Research, U.S. Food and Drug Administration, Silver Spring, MD, USA.

Arifa S KhanLaboratory of Retroviruses, Division of Viral Products, Office of Vaccines Research and Review, Center for Biologics Evaluation and Research, U.S. Food and Drug Administration, Silver Spring, MD, USA. arifa.khan@fda.hhs.gov.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A virus stock of a cloned infectious DNA of simian foamy virus serotype 1 (SFV-1) was obtained in M. dunni cells (designated as pSFV-MD). The kinetics of replication were similar to the parent uncloned SFV-MD stock in M. dunni cells and in FRhK-4 cells, but in Vero cells, pSFV-MD showed a similar level of reverse transcriptase activity, but a faster progression of CPE. HTS variant analysis of pSFV-MD and SFV-MD passaged through Vero cells showed accumulation of G-to-A mutations in the bel2 and tas genes. Some of the mutations created stop codons, truncating Tas and Bet proteins. The generation of genomic variants in SFV-1 was identified by passaging the pSFV-MD genetically homogenous virus stock in Vero cells. The accumulation of APOBEC SNVs in the SFV-1 genome highlights the species-specific interactions of Bet and APOBEC proteins since the macaque SFV-1 Bet could not effectively counteract the AGM APOBEC activity in Vero cells.

Identifiers

PMID41326689

DOI10.1038/s44298-025-00162-5

PMCPMC12669682

What Socratic holds

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LicenceCC BY

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.