ArticleBMC genomics2025
A mannitol-based buffer improves single-cell RNA sequencing of high-salt marine cells.
Article in BMC genomics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Comprehensive guide for optimizing octopus immune cell preparation to enhance single cell RNA sequencing success.Scientific reports · 2026Article
- Neural signaling contributes to heart formation and growth in the invertebrate chordate, Ciona robusta.PLoS biology · 2026Article
- Divergence of immune cell types in chordate blood.Current biology : CB · 2025Article
- Multi-step genomics on single cells and live cultures in sub-nanoliter capsules.bioRxiv : the preprint server for biology · 2025Article
- Immune cell type divergence in a basal chordate.bioRxiv : the preprint server for biology · 2025Article
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Abstract
Single-cell RNA sequencing (scRNA-seq) enables discovery of novel cell states by transcriptomic profiling with minimal prior knowledge, making it useful for studying non-model organisms. For most marine organisms, however, cells are viable at a higher salinity than is compatible with scRNA-seq, impacting data quality and cell representation. We show that a low-salinity phosphate buffer supplemented with D-mannitol (PBS-M) enables higher-quality scRNA-seq of blood cells from the tunicate Ciona robusta. Using PBS-M reduces cell death and ambient mRNA, revealing cell states not otherwise detected. We additionally validate PBS-M in a second tunicate species. This simple protocol modification could enable or improve scRNA-seq for many marine organisms.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.