Evidence map›Paper›PMID 41327378›Full record

ArticleBMC research notes2025

SaSi qPCR: a novel duplex qPCR assay for rapid and simultaneous detection of Streptococcus agalactiae and Streptococcus iniae in fish.

Suwimon Taengphu, Ha Thanh Dong, Watcharachai Meemetta, Saengchan Senapin

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC research notes, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Suwimon TaengphuDepartment of Food, Agriculture and Bioresources, Aquaculture and Aquatic Resources Management Program, School of Environment, Resources and Development, Asian Institute of Technology (AIT), Klong Luang, Pathum Thani, Thailand.
Ha Thanh DongDepartment of Food, Agriculture and Bioresources, Aquaculture and Aquatic Resources Management Program, School of Environment, Resources and Development, Asian Institute of Technology (AIT), Klong Luang, Pathum Thani, Thailand.
Watcharachai MeemettaFish Health Platform, Center of Excellence for Shrimp Molecular Biology and Biotechnology (Centex Shrimp), Faculty of Science, Mahidol University, Bangkok, Thailand.
Saengchan SenapinFish Health Platform, Center of Excellence for Shrimp Molecular Biology and Biotechnology (Centex Shrimp), Faculty of Science, Mahidol University, Bangkok, Thailand. saengchan@biotec.or.th.

Funding

The Budget Bureau and the National Science and Technology Development Agency P-24-51453
6 · The paper itself

Abstract

objectiveTo develop, optimize, and validate a novel duplex quantitative PCR (qPCR) assay, termed SaSi qPCR, for the simultaneous detection of Streptococcus agalactiae (Sa) and Streptococcus iniae (Si) in fish samples.

resultsThe SaSi qPCR assay successfully detected DNA from bacterial cultures and fish specimens, including tilapia (Oreochromis sp.) and Asian sea bass (Lates calcarifer). The method achieved a detection limit of 20 copies per reaction for both targets, with no cross-reactivity observed with other tested fish pathogens. The assay was applied to clinical fish specimens and detected S. agalactiae, S. iniae, and co-infections in fish tissues.

Indexed as

Fish DiseasesReal-Time Polymerase Chain ReactionStreptococcal InfectionsStreptococcus agalactiaeStreptococcus iniaeAnimalsBassDNA, BacterialFishesTilapiaDNA, BacterialBacteriaDiagnosisDuplex qPCRFishStreptococcosis

Identifiers

PMID41327378
PMCPMC12777442

What Socratic holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.