Evidence map›Paper›PMID 41327470›Full record

ArticleStem cell research & therapy2025

Phenotypes in Brugada syndrome with different genotypes triggered by fever or inflammation using gene-edited iPSCs.

Yingrui Li, Lena Rose, Timo Prädel, Mandy Kleinsorge, Xuehui Fan, Zenghui Meng, Chen Yan, Rui Liu, Xinhao Lei, Binyi Zhao and 23 more

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Article in Stem cell research & therapy, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

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4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

33 authors.

Yingrui Li *First Department of Medicine, Faculty of Medicine Mannheim, University Medical Centre Mannheim (UMM), Heidelberg University, Theodor-Kutzer-Ufer 1-3, 68167, Mannheim, Germany.
Lena Rose *First Department of Medicine, Faculty of Medicine Mannheim, University Medical Centre Mannheim (UMM), Heidelberg University, Theodor-Kutzer-Ufer 1-3, 68167, Mannheim, Germany.
Timo Prädel *First Department of Medicine, Faculty of Medicine Mannheim, University Medical Centre Mannheim (UMM), Heidelberg University, Theodor-Kutzer-Ufer 1-3, 68167, Mannheim, Germany.
Mandy KleinsorgeStem Cell Unit, Clinic for Cardiology and Pneumology, University Medical Center Göttingen, 37075, Göttingen, Germany.
Xuehui FanFirst Department of Medicine, Faculty of Medicine Mannheim, University Medical Centre Mannheim (UMM), Heidelberg University, Theodor-Kutzer-Ufer 1-3, 68167, Mannheim, Germany.
Zenghui MengFirst Department of Medicine, Faculty of Medicine Mannheim, University Medical Centre Mannheim (UMM), Heidelberg University, Theodor-Kutzer-Ufer 1-3, 68167, Mannheim, Germany.
Chen YanFirst Department of Medicine, Faculty of Medicine Mannheim, University Medical Centre Mannheim (UMM), Heidelberg University, Theodor-Kutzer-Ufer 1-3, 68167, Mannheim, Germany.
Rui LiuFirst Department of Medicine, Faculty of Medicine Mannheim, University Medical Centre Mannheim (UMM), Heidelberg University, Theodor-Kutzer-Ufer 1-3, 68167, Mannheim, Germany.
Xinhao LeiFirst Department of Medicine, Faculty of Medicine Mannheim, University Medical Centre Mannheim (UMM), Heidelberg University, Theodor-Kutzer-Ufer 1-3, 68167, Mannheim, Germany.
Binyi ZhaoFirst Department of Medicine, Faculty of Medicine Mannheim, University Medical Centre Mannheim (UMM), Heidelberg University, Theodor-Kutzer-Ufer 1-3, 68167, Mannheim, Germany.
Guoqiang YangFirst Department of Medicine, Faculty of Medicine Mannheim, University Medical Centre Mannheim (UMM), Heidelberg University, Theodor-Kutzer-Ufer 1-3, 68167, Mannheim, Germany.
Zhenxing LiaoFirst Department of Medicine, Faculty of Medicine Mannheim, University Medical Centre Mannheim (UMM), Heidelberg University, Theodor-Kutzer-Ufer 1-3, 68167, Mannheim, Germany.
Hendrik DinkelFirst Department of Medicine, Faculty of Medicine Mannheim, University Medical Centre Mannheim (UMM), Heidelberg University, Theodor-Kutzer-Ufer 1-3, 68167, Mannheim, Germany.
Alexandra Viktoria BusleyStem Cell Unit, Clinic for Cardiology and Pneumology, University Medical Center Göttingen, 37075, Göttingen, Germany.
Rujia ZhongFirst Department of Medicine, Faculty of Medicine Mannheim, University Medical Centre Mannheim (UMM), Heidelberg University, Theodor-Kutzer-Ufer 1-3, 68167, Mannheim, Germany.
Feng ZhangFirst Department of Medicine, Faculty of Medicine Mannheim, University Medical Centre Mannheim (UMM), Heidelberg University, Theodor-Kutzer-Ufer 1-3, 68167, Mannheim, Germany.
Qiang XuFirst Department of Medicine, Faculty of Medicine Mannheim, University Medical Centre Mannheim (UMM), Heidelberg University, Theodor-Kutzer-Ufer 1-3, 68167, Mannheim, Germany.
Lasse Maywald *First Department of Medicine, Faculty of Medicine Mannheim, University Medical Centre Mannheim (UMM), Heidelberg University, Theodor-Kutzer-Ufer 1-3, 68167, Mannheim, Germany.
Assem AweimerDepartment of Cardiology and Angiology, Bergmannsheil University Hospitals, Ruhr University of Bochum, 44789, Bochum, Germany.
Mengying HuangFirst Department of Medicine, Faculty of Medicine Mannheim, University Medical Centre Mannheim (UMM), Heidelberg University, Theodor-Kutzer-Ufer 1-3, 68167, Mannheim, Germany.
Alexander Moscu-GregorCenter for Human Genetics and Laboratory Medicine, Martinsried, Germany.
Nazha HamdaniDepartment of Cellular and Translational Physiology, Institute of Physiology, Ruhr University, Bochum, Germany.
Luca SchneiderDepartment of Cellular and Translational Physiology, Institute of Physiology, Ruhr University, Bochum, Germany.
Yeweynwuha ZemediDepartment of Cellular and Translational Physiology, Institute of Physiology, Ruhr University, Bochum, Germany.
Saltanat ZhazykbayevaDepartment of Cellular and Translational Physiology, Institute of Physiology, Ruhr University, Bochum, Germany.
Alyssa HohnFirst Department of Medicine, Faculty of Medicine Mannheim, University Medical Centre Mannheim (UMM), Heidelberg University, Theodor-Kutzer-Ufer 1-3, 68167, Mannheim, Germany.
Zhen YangFirst Department of Medicine, Faculty of Medicine Mannheim, University Medical Centre Mannheim (UMM), Heidelberg University, Theodor-Kutzer-Ufer 1-3, 68167, Mannheim, Germany.
Lin QiaoFirst Department of Medicine, Faculty of Medicine Mannheim, University Medical Centre Mannheim (UMM), Heidelberg University, Theodor-Kutzer-Ufer 1-3, 68167, Mannheim, Germany.
Andreas MüggeDepartment of Cardiology and Angiology, Bergmannsheil University Hospitals, Ruhr University of Bochum, 44789, Bochum, Germany.
Lukas CyganekStem Cell Unit, Clinic for Cardiology and Pneumology, University Medical Center Göttingen, 37075, Göttingen, Germany.
Xiaobo ZhouFirst Department of Medicine, Faculty of Medicine Mannheim, University Medical Centre Mannheim (UMM), Heidelberg University, Theodor-Kutzer-Ufer 1-3, 68167, Mannheim, Germany. xiaobo.zhou@medma.uni-heidelberg.de.ORCID http://orcid.org/0000-0002-5895-0127
Ibrahim Akin *First Department of Medicine, Faculty of Medicine Mannheim, University Medical Centre Mannheim (UMM), Heidelberg University, Theodor-Kutzer-Ufer 1-3, 68167, Mannheim, Germany.
Ibrahim El-Battrawy *Department of Cardiology and Angiology, Bergmannsheil University Hospitals, Ruhr University of Bochum, 44789, Bochum, Germany.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundFever or inflammation state may enhance the Brugada syndrome (BrS) phenotype in some but not all patients. However, the underlying mechanism in human cardiomyocytes has not yet been clarified.

methodsHuman induced pluripotent stem cell (hiPSC) lines generated from fibroblasts of three BrS patients harboring variants in SCN10A (abbreviated as BrS1) and CACNB2 (abbreviated as BrS2), SCN5A (abbreviated as BrS3) and one healthy donor (abbreviated as WT) and a site-corrected (using CRISPR/Cas9) hiPSC line of each BrS patient (abbreviated as isogenic1, isogenic2 and isogenic3) were used for differentiation into cardiomyocytes (hiPSC-CMs). Western blot, patch clamp and calcium transient analyses were carried out.

resultsAll 3 BrS cell lines showed a significantly reduced peak sodium current (I

conclusionsHyperthermia exacerbated the BrS phenotype in hiPSC-CMs carrying SCN10A and SCN5A variants, whereas LPS aggravated the phenotype in all three BrS variants through distinct mechanisms; Hyperthermia and LPS effects on BrS phenotype may be genotype-dependent.

Indexed as

Brugada SyndromeFeverInduced Pluripotent Stem CellsInflammationCell DifferentiationCell LineGene EditingGenotypeHumansLipopolysaccharidesMaleMyocytes, CardiacNAV1.5 Voltage-Gated Sodium ChannelNAV1.8 Voltage-Gated Sodium ChannelPhenotypeLipopolysaccharidesNAV1.5 Voltage-Gated Sodium ChannelNAV1.8 Voltage-Gated Sodium ChannelSCN10A protein, humanSCN5A protein, humanBrugada syndromeHyperthermiaInduced pluripotent stem cellsInflammation

Identifiers

PMID41327470
PMCPMC12670799

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